{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T22:17:09Z","timestamp":1792534629826,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T00:00:00Z","timestamp":1392854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"Summary<\/jats:title>\n \n In an acetate\u2010fed anaerobic\u2013aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (\n EBPR<\/jats:styled-content>\n ),\n \n D<\/jats:italic>\n <\/jats:styled-content>\n efluviicoccus<\/jats:italic>\n \u2010related tetrad\u2010forming organisms (\n DTFO<\/jats:styled-content>\n ) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant\n DTFO<\/jats:styled-content>\n , \u2018\n \n C<\/jats:italic>\n <\/jats:styled-content>\n andidatus<\/jats:italic>\n \u2005\n D<\/jats:styled-content>\n efluviicoccus tetraformis strain\n TFO<\/jats:styled-content>\n 71\u2019, was successfully constructed and characterized. Examining the genome confirmed the presence of genes related to the synthesis and degradation of glycogen and polyhydroxyalkanoate (\n PHA<\/jats:styled-content>\n ), which function as energy and carbon storage compounds.\n TFO<\/jats:styled-content>\n 71 and \u2018\n \n C<\/jats:italic>\n <\/jats:styled-content>\n andidatus<\/jats:italic>\n \u2005\n A<\/jats:styled-content>\n ccumulibacter phosphatis\u2019 (\n CAP<\/jats:styled-content>\n )\n UW<\/jats:styled-content>\n \u20101 and\n CAP UW<\/jats:styled-content>\n \u20102, representative polyphosphate\u2010accumulating organisms (\n PAO<\/jats:styled-content>\n ), have\n PHA<\/jats:styled-content>\n metabolism\u2010related genes with high homology, but\n TFO<\/jats:styled-content>\n 71 has unique genes for\n PHA<\/jats:styled-content>\n synthesis, gene regulation and granule management. We further discovered genes encoding\n DTFO<\/jats:styled-content>\n polyphosphate (\n polyP<\/jats:styled-content>\n ) synthesis, suggesting that\n TFO<\/jats:styled-content>\n 71 may synthesize\n polyP<\/jats:styled-content>\n under untested conditions. However,\n TFO<\/jats:styled-content>\n 71 may not activate these genes under\n EBPR<\/jats:styled-content>\n conditions because the retrieved genome does not contain all inorganic phosphate transporters that are characteristic of\n PAOs<\/jats:styled-content>\n (\n CAP UW<\/jats:styled-content>\n \u20101,\n CAP UW<\/jats:styled-content>\n \u20102,\n \n M<\/jats:italic>\n <\/jats:styled-content>\n icrolunatus phosphovorus<\/jats:italic>\n \u2005\n NM<\/jats:styled-content>\n \u20101 and\n \n T<\/jats:italic>\n <\/jats:styled-content>\n etrasphaera<\/jats:italic>\n species). As a first step in characterizing\n EBPR<\/jats:styled-content>\n \u2010associated\n DTFO<\/jats:styled-content>\n metabolism, this study identifies important differences between\n DTFO<\/jats:styled-content>\n and\n PAO<\/jats:styled-content>\n that may contribute to\n EBPR<\/jats:styled-content>\n community competition and deterioration.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12383","type":"journal-article","created":{"date-parts":[[2014,1,15]],"date-time":"2014-01-15T21:53:50Z","timestamp":1389822830000},"page":"2739-2751","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Metagenomic characterization of \u2018\n \n C<\/i>\n <\/scp>\n andidatus<\/i>\n \u2005\n D<\/scp>\n efluviicoccus tetraformis strain\n TFO<\/scp>\n 71\u2019, a tetrad\u2010forming organism, predominant in an anaerobic\u2013aerobic membrane bioreactor with deteriorated biological phosphorus removal"],"prefix":"10.1111","volume":"16","author":[{"given":"Masaru K.","family":"Nobu","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering University of Illinois at Urbana\u2010Champaign 205 North Mathews Ave Urbana IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Tamaki","sequence":"additional","affiliation":[{"name":"Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Central 6, Higashi 1\u20101\u20101 Tsukuba Ibaraki 305\u20138566 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kengo","family":"Kubota","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering Tohoku University 6\u20106\u201006 Aza\u2010Aoba, Aramaki, Aoba\u2010ku Sendai Miyagi 980\u20138579 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Tso","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering University of Illinois at Urbana\u2010Champaign 205 North Mathews Ave Urbana IL 61801 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