{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:40:32Z","timestamp":1789180832316,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,2,24]],"date-time":"2014-02-24T00:00:00Z","timestamp":1393200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"publisher","award":["102 101 0025"],"award-info":[{"award-number":["102 101 0025"]}],"id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001381","name":"National Research Foundation-Prime Minister's office, Republic of Singapore","doi-asserted-by":"publisher","award":["NRFCRP05200905"],"award-info":[{"award-number":["NRFCRP05200905"]}],"id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Summary<\/jats:title>\n \n 1,1,1\u2010Trichloroethane (\n TCA<\/jats:styled-content>\n ) and chloroform are two notorious groundwater pollutants. Here we report the isolation and characterization of\n \n D<\/jats:italic>\n <\/jats:styled-content>\n esulfitobacterium<\/jats:italic>\n sp. strain\n PR<\/jats:styled-content>\n that rapidly dechlorinates both compounds. In pyruvate\u2010amended medium, strain\n PR<\/jats:styled-content>\n reductively dechlorinates \u223c\u20091.0\u2009mM\n TCA<\/jats:styled-content>\n completely to monochloroethane within 15 days. Under the same conditions, strain\n PR<\/jats:styled-content>\n dechlorinates \u223c\u20091.2\u2009mM chloroform to predominantly dichloromethane (\u223c\u20091.14\u2009mM) and trace amount of monochloromethane (\u223c\u20090.06\u2009mM) within 10 days. Strain\n PR<\/jats:styled-content>\n shares 96.7% 16\n S rRNA<\/jats:styled-content>\n gene sequence similarity with its closest relative \u2013\n \n D<\/jats:italic>\n <\/jats:styled-content>\n esulfitobacterium metallireducens<\/jats:italic>\n strain 853\u201015; however, it distinguishes itself from known\n \n D<\/jats:italic>\n <\/jats:styled-content>\n esulfitobacterium<\/jats:italic>\n strains by its inability of utilizing several of their commonly shared substrates such as lactate, thiosulfate and sulfite. A reductive dehalogenase gene (\n \n ctrA<\/jats:italic>\n <\/jats:styled-content>\n ) in strain\n PR<\/jats:styled-content>\n was identified to be responsible for dechlorination of both\n TCA<\/jats:styled-content>\n and chloroform, showing a maximum expression level of 5.95\u2009\u223c\u20096.25 copies of transcripts cell\n \u20101<\/jats:sup>\n .\n CtrA<\/jats:styled-content>\n shares 94% amino acid sequence identity with\n CfrA<\/jats:styled-content>\n in\n \n D<\/jats:italic>\n <\/jats:styled-content>\n ehalobacter<\/jats:italic>\n sp. strain\n CF<\/jats:styled-content>\n 50 and\n DcrA<\/jats:styled-content>\n in\n \n D<\/jats:italic>\n <\/jats:styled-content>\n ehalobacter<\/jats:italic>\n sp. strain\n DCA<\/jats:styled-content>\n . Interestingly, strain\n PR<\/jats:styled-content>\n could tolerate high aqueous concentrations (up to 0.45\u2009mM) of trichloroethene, another groundwater pollutant that often coexists with\n TCA<\/jats:styled-content>\n \/chloroform. As the first chloroform\u2010respiring and the second\n TCA<\/jats:styled-content>\n \u2010respiring isolate that has been identified,\n \n D<\/jats:italic>\n <\/jats:styled-content>\n esulfitobacterium<\/jats:italic>\n sp. strain\n PR<\/jats:styled-content>\n may prove useful in remediation of halogenated alkanes with trihalomethyl (\u2010\n \n CX\n 3<\/jats:sub>\n <\/jats:styled-content>\n ) groups.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12387","type":"journal-article","created":{"date-parts":[[2014,1,15]],"date-time":"2014-01-15T21:53:51Z","timestamp":1389822831000},"page":"3387-3397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["A\n \n D<\/i>\n <\/scp>\n esulfitobacterium<\/i>\n sp. strain\n PR<\/scp>\n reductively dechlorinates both 1,1,1\u2010trichloroethane and chloroform"],"prefix":"10.1111","volume":"16","author":[{"given":"Chang","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es990809f"},{"key":"e_1_2_7_3_1","unstructured":"ATSDR(2006)Toxicological profile for 1 1 1\u2010trichloroethane."},{"key":"e_1_2_7_4_1","unstructured":"ATSDR(2011)The ATSDR 2011 Substance Priority List. 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