{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:24:58Z","timestamp":1790364298575,"version":"4.1.0"},"reference-count":83,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T00:00:00Z","timestamp":1399420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"Summary<\/jats:title>\n \n The dematiaceous (melanized) fungus\n \n S<\/jats:italic>\n <\/jats:styled-content>\n cedosporium prolificans<\/jats:italic>\n is an emerging and frequently fatal pathogen of immunocompromised humans and which, along with the closely related fungi\n \n P<\/jats:italic>\n <\/jats:styled-content>\n seudallescheria boydii<\/jats:italic>\n ,\n \n S<\/jats:italic>\n <\/jats:styled-content>\n cedosporium apiospermum<\/jats:italic>\n and\n \n S<\/jats:italic>\n <\/jats:styled-content>\n .\u2009aurantiacum<\/jats:italic>\n in the\n \n P<\/jats:italic>\n <\/jats:styled-content>\n seudallescheria<\/jats:italic>\n \u2013\n \n S<\/jats:italic>\n <\/jats:styled-content>\n cedosporium<\/jats:italic>\n complex, is a contributing aetiology to tsunami lung and central nervous system infections in near\u2010drowning victims who have aspirated water laden with spores. At present, the natural habitat of the fungus is largely unknown, and accurate detection methods are needed to identify environmental reservoirs of infectious propagules. In this study, we report the development of a monoclonal antibody (m\n A<\/jats:styled-content>\n b) (\n CA<\/jats:styled-content>\n 4) specific to\n \n S<\/jats:italic>\n <\/jats:styled-content>\n .\u2009prolificans<\/jats:italic>\n , which does not cross\u2010react with closely related fungi in the\n \n P<\/jats:italic>\n <\/jats:styled-content>\n seudallescheria<\/jats:italic>\n \u2013\n \n S<\/jats:italic>\n <\/jats:styled-content>\n cedosporium<\/jats:italic>\n complex or with a wide range of mould and yeast species pathogenic to humans. Using genome sequencing of a soil isolate and targeted gene disruption of the\n CA<\/jats:styled-content>\n 4 antigen\u2010encoding gene, we show that m\n A<\/jats:styled-content>\n b\n CA<\/jats:styled-content>\n 4 binds to the melanin\u2010biosynthetic enzyme tetrahydroxynaphthalene reductase. Enzyme\u2010deficient mutants produce orange\u2010brown or green\u2010brown spore suspensions compared with the black spore suspension of the wild\u2010type strain. Using m\n A<\/jats:styled-content>\n b\n CA<\/jats:styled-content>\n 4 and a m\n A<\/jats:styled-content>\n b (\n HG<\/jats:styled-content>\n 12) specific to the related fungi\n \n P<\/jats:italic>\n <\/jats:styled-content>\n .\u2009boydii<\/jats:italic>\n ,\n \n P<\/jats:italic>\n <\/jats:styled-content>\n .\u2009apiosperma<\/jats:italic>\n ,\n \n S<\/jats:italic>\n <\/jats:styled-content>\n .\u2009apiospermum<\/jats:italic>\n and\n \n S<\/jats:italic>\n <\/jats:styled-content>\n .\u2009aurantiacum<\/jats:italic>\n , we demonstrate how the m\n A<\/jats:styled-content>\n bs can be used in combination with a semiselective isolation procedure to track these opportunistic pathogens in environmental samples containing mixed populations of human pathogenic fungi. Specificity of m\n A<\/jats:styled-content>\n b\n CA<\/jats:styled-content>\n 4 was confirmed by sequencing of the internally transcribed spacer 1 (\n ITS<\/jats:styled-content>\n 1)\u20105.8\n S\u2010ITS<\/jats:styled-content>\n 2 r\n RNA<\/jats:styled-content>\n \u2010encoding regions of fungi isolated from estuarine muds.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12470","type":"journal-article","created":{"date-parts":[[2014,3,31]],"date-time":"2014-03-31T02:57:34Z","timestamp":1396234654000},"page":"1023-1038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identifying the emerging human pathogen\n \n S<\/i>\n <\/scp>\n cedosporium prolificans<\/i>\n by using a species\u2010specific monoclonal antibody that binds to the melanin biosynthetic enzyme tetrahydroxynaphthalene reductase"],"prefix":"10.1111","volume":"17","author":[{"given":"Christopher 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