{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:41:10Z","timestamp":1789771270716,"version":"4.0.1"},"reference-count":70,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T00:00:00Z","timestamp":1400112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Flemish Fund for Scientific Research","award":["FWO11\/KAN\/043"],"award-info":[{"award-number":["FWO11\/KAN\/043"]}]},{"name":"Flemish Fund for Scientific Research","award":["FWO11\/PDO\/084"],"award-info":[{"award-number":["FWO11\/PDO\/084"]}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Summary<\/jats:title>\n \n Several\n G<\/jats:styled-content>\n ram\u2010positive bacteria carry genes for anaerobic reduction of NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n via NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n to NH\n 4<\/jats:sub>\n +<\/jats:sup>\n or gaseous nitrogen compounds, but the processes are understudied for these organisms. Here, we present results from a whole\u2010genome analysis of the soil bacterium\n \n B<\/jats:italic>\n <\/jats:styled-content>\n acillus vireti<\/jats:italic>\n and a phenotypic characterization of intermediate and end\u2010products, formed under anoxic conditions in the presence of NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n .\n \n B<\/jats:italic>\n <\/jats:styled-content>\n acillus vireti<\/jats:italic>\n has a versatile metabolism. It produces acetate, formate, succinate and lactate from fermentation and performs dissimilatory nitrate reduction via NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n to ammonium (\n DNRA<\/jats:styled-content>\n ) using\n NrfA<\/jats:styled-content>\n , while\n NirB<\/jats:styled-content>\n may detoxify\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n in the cytoplasm. Moreover, it produces\n \n NO<\/jats:roman>\n <\/jats:styled-content>\n from an unknown source and reduces it via\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n to\n \n \n N\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n using two enzymes connected to denitrification: an unusual NO reductase,\n \n qCu\n A<\/jats:sub>\n Nor\n <\/jats:styled-content>\n encoded by\n cbaA<\/jats:italic>\n , and a z\u2010type\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n reductase, encoded by\n \n nosZ<\/jats:italic>\n <\/jats:styled-content>\n . In batch cultures,\n \n B<\/jats:italic>\n <\/jats:styled-content>\n .\u2009vireti<\/jats:italic>\n reduced all\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n to\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n before the\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n was reduced further. The quantities of all products varied with the initial\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n concentration. With 5\u2009m\n \n M\n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n , 90% was reduced to\n \n \n NH\n 4<\/jats:sub>\n +<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n while with \u2265\u200920\u2009m\n \n M\n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n , 50% was reduced to\n \n NO<\/jats:roman>\n <\/jats:styled-content>\n ,\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n and\n \n \n N\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n . This organism is thus an aggressive\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n accumulator and may act as a net source and sink of\n \n NO<\/jats:roman>\n <\/jats:styled-content>\n and\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/1462-2920.12478","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T03:55:07Z","timestamp":1396929307000},"page":"3196-3210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["The nitrate\u2010ammonifying and\n \n nosZ<\/i>\n <\/scp>\n \u2010carrying bacterium\n \n B<\/i>\n <\/scp>\n acillus vireti<\/i>\n is a potent source and sink for nitric and nitrous oxide under high nitrate conditions"],"prefix":"10.1111","volume":"16","author":[{"given":"Daniel","family":"Mania","sequence":"first","affiliation":[{"name":"Department of Chemistry, Biotechnology and Food Science Norwegian University of Life Science \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Heylen","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Department of Biochemistry and Microbiology University of Ghent Gent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob J. M.","family":"van Spanning","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology Faculty of Earth and Life Science VU University Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5sa","family":"Frosteg\u00e5rd","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biotechnology and Food Science Norwegian University of Life Science \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20110716"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0321"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzh037"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-167"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2007.01557.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00608-10"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1128\/aem.44.6.1342-1348.1982","article-title":"Nitrous oxide production by organisms other than 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