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Here, we describe the role of\n                    <jats:italic>clpC<\/jats:italic>\n                    operon in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>acillus anthracis<\/jats:italic>\n                    . We generated knockout strains of the\n                    <jats:italic>clpC<\/jats:italic>\n                    operon genes to investigate the impact of\n                    <jats:styled-content style=\"fixed-case\">CtsR<\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">McsA<\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">McsB<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">ClpC<\/jats:styled-content>\n                    deletion on essential processes of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009anthracis.<\/jats:italic>\n                    We observed that growth, cell division, sporulation and germination were severely affected in\n                    <jats:italic>mcsB<\/jats:italic>\n                    and\n                    <jats:italic>clpC<\/jats:italic>\n                    deleted strains, while none of deletions affected toxin secretion. Growth defect in these strains was pronounced at elevated temperature. The growth pattern gets restored on complementation of\n                    <jats:italic>mcsB<\/jats:italic>\n                    and\n                    <jats:italic>clpC<\/jats:italic>\n                    in respective mutants. Electron microscopic examination revealed that\n                    <jats:italic>mcsB<\/jats:italic>\n                    and\n                    <jats:italic>clpC<\/jats:italic>\n                    deletion also causes defect in septum formation leading to cell elongation. These vegetative cell deformities were accompanied by inability of mutant strains to generate morphologically intact spores. Higher levels of polyhydroxybutyrate granules accumulation were also observed in these deletion strains, indicating a defect in sporulation process. Our results demonstrate, for the first time, the vital role played by\n                    <jats:styled-content style=\"fixed-case\">McsB<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">ClpC<\/jats:styled-content>\n                    in physiology of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009anthracis<\/jats:italic>\n                    and open up further interest on this operon, which might be of importance to success of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\u2009\n                    <jats:italic>anthracis<\/jats:italic>\n                    as pathogen.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12548","type":"journal-article","created":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T00:19:56Z","timestamp":1403223596000},"page":"855-865","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["<i>clpC<\/i>\n                    operon regulates cell architecture and sporulation in\n                    <i>Bacillus anthracis<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Lalit K.","family":"Singh","sequence":"first","affiliation":[{"name":"CSIR\u2010Institute of Genomics and Integrative Biology  Delhi 110007 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Dhasmana","sequence":"additional","affiliation":[{"name":"CSIR\u2010Institute of Genomics and Integrative Biology  Delhi 110007 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