{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:43:02Z","timestamp":1791182582542,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T00:00:00Z","timestamp":1410739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"Summary<\/jats:title>\n \n \n L<\/jats:italic>\n <\/jats:styled-content>\n egionella pneumophila<\/jats:italic>\n is a pathogenic bacterium commonly found in water. Eventually, it could be transmitted to humans via inhalation of contaminated aerosols. Iron is known as a key requirement for the growth of\n \n L<\/jats:italic>\n <\/jats:styled-content>\n .\u2009pneumophila<\/jats:italic>\n in the environment and within its hosts. Many studies were performed to understand iron utilization by\n \n L<\/jats:italic>\n <\/jats:styled-content>\n .\u2009pneumophila<\/jats:italic>\n but no global approaches were conducted. In this study, transcriptomic analyses were performed, comparing gene expression in\n \n L<\/jats:italic>\n <\/jats:styled-content>\n .\u2009pneumophila<\/jats:italic>\n in standard versus iron restricted conditions. Among the regulated genes, a newly described one,\n lpp_2867<\/jats:italic>\n , was highly induced in iron\u2010restricted conditions. Mutants lacking this gene in\n \n L<\/jats:italic>\n <\/jats:styled-content>\n .\u2009pneumophila<\/jats:italic>\n were not affected in siderophore synthesis or utilization. On the contrary, they were defective for growth on iron\u2010depleted solid media and for ferrous iron uptake. A sequence analysis predicts that\n L<\/jats:styled-content>\n pp_2867 is a membrane protein, suggesting that it is involved in ferrous iron transport. We thus named it\n IroT<\/jats:styled-content>\n , for iron transporter. Infection assays showed that the mutants are highly impaired in intracellular growth within their environmental host\n \n A<\/jats:italic>\n <\/jats:styled-content>\n canthamoeba castellanii<\/jats:italic>\n and human macrophages. Taken together, our results show that IroT is involved, directly or indirectly, in ferrous iron transport and is a key virulence factor for\n \n L<\/jats:italic>\n <\/jats:styled-content>\n .\u2009pneumophila<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/1462-2920.12604","type":"journal-article","created":{"date-parts":[[2014,8,20]],"date-time":"2014-08-20T22:04:38Z","timestamp":1408572278000},"page":"1338-1350","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["\n IroT\/mavN<\/i>\n <\/scp>\n , a new iron\u2010regulated gene involved in\n \n L<\/i>\n <\/scp>\n egionella pneumophila<\/i>\n virulence against amoebae and macrophages"],"prefix":"10.1111","volume":"17","author":[{"given":"Emilie","family":"Portier","sequence":"first","affiliation":[{"name":"Laboratoire Ecologie et Biologie des Interactions UMR CNRS 7267 Equipe Microbiologie de l'Eau Universit\u00e9 de Poitiers Poitiers 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