{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:27:07Z","timestamp":1793060827504,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,1,7]],"date-time":"2015-01-07T00:00:00Z","timestamp":1420588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Summary<\/jats:title>\n \n Cyanobacteria possess\n CO<\/jats:styled-content>\n 2<\/jats:sub>\n \u2010concentrating mechanisms (\n CCM<\/jats:styled-content>\n ) that functionally compensate for the poor affinity of their ribulose\u20101,5\u2010bisphosphate carboxylase\/oxygenase (\n R<\/jats:styled-content>\n ubisco) to\n CO<\/jats:styled-content>\n 2<\/jats:sub>\n . It was proposed that 2\u2010phosphoglycolate (2\n PG<\/jats:styled-content>\n ), produced by the oxygenase activity of\n R<\/jats:styled-content>\n ubisco and metabolized via photorespiratory routes, serves as a signal molecule for the induction of\n CCM<\/jats:styled-content>\n \u2010related genes under limiting CO\n 2<\/jats:sub>\n level (\n LC<\/jats:styled-content>\n ) conditions. However,\n in vivo<\/jats:italic>\n evidence is still missing. Since 2\n PG<\/jats:styled-content>\n does not permeate the cells, we manipulated its internal concentration. Four putative phosphoglycolate phosphatases (\n PGP<\/jats:styled-content>\n ases) encoding genes (\n \n slr0458<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n sll1349<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n slr0586<\/jats:italic>\n <\/jats:styled-content>\n and\n \n slr1762<\/jats:italic>\n <\/jats:styled-content>\n ) were identified in the cyanobacterium\n \n S<\/jats:italic>\n <\/jats:styled-content>\n ynechocystis<\/jats:italic>\n \u2005\n PCC<\/jats:styled-content>\n 6803. Expression of\n \n slr0458<\/jats:italic>\n <\/jats:styled-content>\n in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n scherichia coli<\/jats:italic>\n led to a significant rise in\n PGP<\/jats:styled-content>\n ase activity. A\n \n S<\/jats:italic>\n <\/jats:styled-content>\n ynechocystis<\/jats:italic>\n mutant overexpressing (\n OE<\/jats:styled-content>\n )\n \n slr0458<\/jats:italic>\n <\/jats:styled-content>\n was constructed. Compared with the wild type (WT), the mutant grew slower under limiting\n CO<\/jats:styled-content>\n 2<\/jats:sub>\n concentration and the intracellular 2\n PG<\/jats:styled-content>\n level was considerably smaller than in the wild type, the transcript abundance of\n LC<\/jats:styled-content>\n \u2010induced genes including\n \n cmpA<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n sbtA<\/jats:italic>\n <\/jats:styled-content>\n and\n \n ndhF3<\/jats:italic>\n <\/jats:styled-content>\n was reduced, and the\n OE<\/jats:styled-content>\n cells acclimated slower to\n LC<\/jats:styled-content>\n \u2013 indicated by the delayed rise in the apparent photosynthetic affinity to inorganic carbon. Data obtained here implicated 2\n PG<\/jats:styled-content>\n in the acclimation of this cyanobacterium to\n LC<\/jats:styled-content>\n but also indicated that other, yet to be identified components, are involved.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12638","type":"journal-article","created":{"date-parts":[[2014,10,8]],"date-time":"2014-10-08T23:31:46Z","timestamp":1412811106000},"page":"1794-1804","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Does 2\u2010phosphoglycolate serve as an internal signal molecule of inorganic carbon deprivation in the cyanobacterium\n \n S<\/i>\n <\/scp>\n ynechocystis<\/i>\n sp.\n PCC<\/scp>\n 6803?"],"prefix":"10.1111","volume":"17","author":[{"given":"Maya","family":"Haimovich\u2010Dayan","sequence":"first","affiliation":[{"name":"Department of Plant and Environmental Sciences Edmond J. Safra Campus The Hebrew University of Jerusalem Jerusalem 91904 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Lieman\u2010Hurwitz","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences Edmond J. Safra Campus The Hebrew University of Jerusalem Jerusalem 91904 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Orf","sequence":"additional","affiliation":[{"name":"Applied Metabolome Analysis Max Planck Institute of Molecular Plant Physiology Am M\u00fchlenberg Potsdam\u2010Golm 114476 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Hagemann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biowissenschaften Abt. Pflanzenphysiologie Universit\u00e4t Rostock Einsteinstra\u00dfe 3 Rostock D\u201018059 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences Edmond J. 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