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Due to lack of sensitive methods for detection of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    in water, this study utilized an alternative surveillance approach for evaluating marine habitat contamination using wild mussels. The objectives of this study were to (\n                    <jats:styled-content style=\"fixed-case\">i<\/jats:styled-content>\n                    ) validate sensitive molecular tools for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    detection in mussels and (\n                    <jats:styled-content style=\"fixed-case\">ii<\/jats:styled-content>\n                    ) apply optimized methods in a surveillance study to determine the prevalence and genotype(s) of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    in mussels. Simplex polymerase chain reaction screening and multiplex genotyping assays were validated and then applied on 959 wild\u2010caught mussels collected from central\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    alifornia. Thirteen mussels (1.4%) had detectable\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    \u2005\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    and the presence of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    in mussels was significantly associated with proximity to freshwater run\u2010off and collection during the wet season. Molecular characterization revealed alleles from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    types\n                    <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\n                    \/\n                    <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">X<\/jats:styled-content>\n                    at the\n                    <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                    1 locus, and a novel atypical\n                    <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                    1 allele that was recently documented in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    \u2010infected carnivores from\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    alifornia. Findings demonstrate higher than previously reported\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009gondii<\/jats:italic>\n                    contamination of\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    alifornia coastlines, and describe novel strains of the parasite that further link terrestrial sources with marine contamination.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12685","type":"journal-article","created":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T22:21:06Z","timestamp":1415053266000},"page":"4177-4188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Surveillance for\n                    <scp>\n                      <i>T<\/i>\n                    <\/scp>\n          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