{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:40:26Z","timestamp":1787013626678,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T00:00:00Z","timestamp":1422316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Basic Research and Development Program","award":["2012CB114004"],"award-info":[{"award-number":["2012CB114004"]}]},{"name":"National Key Basic Research and Development Program","award":["2012CB114102"],"award-info":[{"award-number":["2012CB114102"]}]},{"DOI":"10.13039\/501100010042","name":"Special Fund for Agro-scientific Research in the Public Interest","doi-asserted-by":"crossref","award":["201303023"],"award-info":[{"award-number":["201303023"]}],"id":[{"id":"10.13039\/501100010042","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China Agriculture Research System","award":["CARS-3-1-15"],"award-info":[{"award-number":["CARS-3-1-15"]}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"Summary<\/jats:title>\n \n \n F<\/jats:italic>\n <\/jats:styled-content>\n usarium<\/jats:italic>\n head blight (\n FHB<\/jats:styled-content>\n ) caused by\n \n F<\/jats:italic>\n <\/jats:styled-content>\n usarium graminearum<\/jats:italic>\n is a devastating disease of cereal crops worldwide. Recently, a novel fungicide\n JS<\/jats:styled-content>\n 399\u201019 has been launched into the marketplace to manage\n FHB<\/jats:styled-content>\n . It is compelling that\n JS<\/jats:styled-content>\n 399\u201019 shows highly inhibitory activity towards some\n \n F<\/jats:italic>\n <\/jats:styled-content>\n usarium<\/jats:italic>\n species, but not to other fungi, indicating that it is an environmentally compatible fungicide. To explore the mode of action of this species\u2010specific compound, we conducted a whole\u2010genome transcript profiling together with genetic and biochemical assays, and discovered that\n JS<\/jats:styled-content>\n 399\u201019 targets the myosin\n I<\/jats:styled-content>\n of\n \n F<\/jats:italic>\n <\/jats:styled-content>\n .\u2009graminearum<\/jats:italic>\n (\n FgM<\/jats:styled-content>\n yo1).\n FgM<\/jats:styled-content>\n yo1 is essential for\n \n F<\/jats:italic>\n <\/jats:styled-content>\n .\u2009graminearum<\/jats:italic>\n growth. A point mutation\n S217L<\/jats:styled-content>\n or\n E420K<\/jats:styled-content>\n in\n FgM<\/jats:styled-content>\n yo1 is responsible for\n \n F<\/jats:italic>\n <\/jats:styled-content>\n .\u2009graminearum<\/jats:italic>\n resistance to\n JS<\/jats:styled-content>\n 399\u201019. In addition, transformation of\n \n F<\/jats:italic>\n <\/jats:styled-content>\n .\u2009graminearum<\/jats:italic>\n with the myosin\n I<\/jats:styled-content>\n gene of\n \n M<\/jats:italic>\n <\/jats:styled-content>\n agnaporthe grisea<\/jats:italic>\n , the causal agent of rice blast, also led to\n JS<\/jats:styled-content>\n 399\u201019 resistance.\n JS<\/jats:styled-content>\n 399\u201019 strongly inhibits the\n ATP<\/jats:styled-content>\n ase activity of the wild\u2010type\n FgM<\/jats:styled-content>\n yo1, but not the mutated\n FgM<\/jats:styled-content>\n yo1\n S217L\/E420K<\/jats:sup>\n . These results provide us a new insight into the design of species\u2010specific antifungal compounds. Furthermore, our strategy can be applied to identify novel drug targets in various pathogenic organisms.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12711","type":"journal-article","created":{"date-parts":[[2014,11,18]],"date-time":"2014-11-18T04:27:28Z","timestamp":1416284848000},"page":"2735-2746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["A small molecule species specifically inhibits\n \n F<\/i>\n <\/scp>\n usarium<\/i>\n myosin\n I<\/scp>"],"prefix":"10.1111","volume":"17","author":[{"given":"Chengqi","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 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