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This work was motivated by the detection in the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009marinus<\/jats:italic>\n                    genome of a gene with high similarity to\n                    <jats:styled-content style=\"fixed-case\">CTP<\/jats:styled-content>\n                    :\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    \u2010\n                    <jats:italic>myo<\/jats:italic>\n                    \u2010inositol\u20101\u2010phosphate cytidylyltransferase, the enzyme that synthesizes cytidine diphosphate (\n                    <jats:styled-content style=\"fixed-case\">CDP<\/jats:styled-content>\n                    )\u2010inositol, a metabolite only known in the synthesis of di\u2010\n                    <jats:italic>myo<\/jats:italic>\n                    \u2010inositol phosphate. However, this solute is absent in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009marinus<\/jats:italic>\n                    . The fate of radiolabelled\n                    <jats:styled-content style=\"fixed-case\">CDP<\/jats:styled-content>\n                    \u2010inositol was investigated in cell extracts to reveal that radioactive inositol was incorporated into the chloroform\u2010soluble fraction. Mass spectrometry showed that the major lipid product has a molecular mass of 810\u2009\n                    <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    a and contains inositol phosphate and alkyl chains attached to glycerol by ether bonds. The occurrence of ether\u2010linked lipids is rare in bacteria and has not been described previously in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009marinus<\/jats:italic>\n                    . The relevant synthase was identified by functional expression of the candidate gene in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>scherichia coli<\/jats:italic>\n                    . The enzyme catalyses the transfer of\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    \u2010\n                    <jats:italic>myo<\/jats:italic>\n                    \u2010inositol\u20101\u2010phosphate from\n                    <jats:styled-content style=\"fixed-case\">CDP<\/jats:styled-content>\n                    \u2010inositol to dialkylether glycerol yielding dialkylether glycerophosphoinositol. Database searching showed homologous proteins in two bacterial classes,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>phingobacteria<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>lphaproteobacteria<\/jats:italic>\n                    . This is the first report of the involvement of\n                    <jats:styled-content style=\"fixed-case\">CDP<\/jats:styled-content>\n                    \u2010inositol in phospholipid synthesis.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12734","type":"journal-article","created":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T18:53:12Z","timestamp":1417805592000},"page":"2492-2504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A novel pathway for the synthesis of inositol phospholipids uses cytidine diphosphate (\n                    <scp>CDP<\/scp>\n                    )\u2010inositol as donor of the polar head group"],"prefix":"10.1111","volume":"17","author":[{"given":"Carla D.","family":"Jorge","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Av. da Rep\u00fablica\u2010EAN Apartado 127 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