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We identified recently a large\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010acyltransferase (\n                    <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\n                    ) polyketide synthase gene cluster responsible for the biosynthesis of the antifungal, anti\u2010oomycete and antitumor haterumalide, oocydin\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    (\n                    <jats:italic>ooc<\/jats:italic>\n                    ). Using genome sequencing and comparative genomics, we show that the\n                    <jats:italic>ooc<\/jats:italic>\n                    gene cluster is widespread within biocontrol and phytopathogenic strains of the enterobacteria,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>erratia<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>ickeya<\/jats:italic>\n                    . The analysis of in frame deletion mutants confirmed the role of a hydroxymethylglutaryl\u2010coenzyme A synthase cassette, three flavin\u2010dependent tailoring enzymes, a free\u2010standing acyl carrier protein and two hypothetical proteins in oocydin\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    biosynthesis. The requirement of the three\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010acting\n                    <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\n                    domains for the biosynthesis of the macrolide was also demonstrated. Expression of the\n                    <jats:italic>ooc<\/jats:italic>\n                    gene cluster was shown to be positively regulated by an\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010acyl\u2010L\u2010homoserine lactone\u2010based quorum sensing system, but operating in a strain\u2010dependent manner. At a post\u2010transcriptional level, the\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    chaperone,\n                    <jats:styled-content style=\"fixed-case\">Hfq<\/jats:styled-content>\n                    , plays a key role in oocydin\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    biosynthesis. The\n                    <jats:styled-content style=\"fixed-case\">Hfq<\/jats:styled-content>\n                    \u2010dependent regulation is partially mediated by the stationary phase sigma factor,\n                    <jats:styled-content style=\"fixed-case\">RpoS<\/jats:styled-content>\n                    , which was also shown to positively regulate the synthesis of the macrolide. Our results reveal differential regulation of the divergently transcribed\n                    <jats:italic>ooc<\/jats:italic>\n                    transcriptional units, highlighting the complexity of oocydin\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    production.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12839","type":"journal-article","created":{"date-parts":[[2015,3,6]],"date-time":"2015-03-06T21:33:18Z","timestamp":1425677598000},"page":"2993-3008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Biosynthesis of the antifungal haterumalide, oocydin\n                    <scp>A<\/scp>\n                    , in\n                    <scp>\n                      <i>S<\/i>\n                    <\/scp>\n                    <i>erratia<\/i>\n                    , and its regulation by quorum sensing,\n                    <scp>RpoS<\/scp>\n                    and\n                    <scp>Hfq<\/scp>"],"prefix":"10.1111","volume":"17","author":[{"given":"Miguel A.","family":"Matilla","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Cambridge  Tennis Court Road Cambridge CB2\u20091QW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Finian J.","family":"Leeper","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Cambridge  Lensfield Road Cambridge CB2\u20091EW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George P. 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