{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:59:53Z","timestamp":1788872393377,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,4,22]],"date-time":"2015-04-22T00:00:00Z","timestamp":1429660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"United States Department of Agriculture (USDA) National Institute of Food and Agriculture","award":["2010-65110-20439"],"award-info":[{"award-number":["2010-65110-20439"]}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2012CB114002"],"award-info":[{"award-number":["2012CB114002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Major Project of Breeding for New Transgenic Organisms","award":["2012ZX08009003"],"award-info":[{"award-number":["2012ZX08009003"]}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"Summary<\/jats:title>\n \n Various surface signals are recognized by\n \n M<\/jats:italic>\n <\/jats:styled-content>\n agnaporthe oryzae<\/jats:italic>\n to activate the\n P<\/jats:styled-content>\n mk1\n MAP<\/jats:styled-content>\n kinase that is essential for appressorium formation and invasive growth. One of upstream sensors of the\n P<\/jats:styled-content>\n mk1 pathway is the\n MoM<\/jats:styled-content>\n sb2 signalling mucin. However, the activation of\n MoM<\/jats:styled-content>\n sb2 and its relationship with other sensors is not clear. In this study, we showed that the cleavage and transmembrane domains are essential for\n MoM<\/jats:styled-content>\n sb2 functions. Cleavage of\n MoM<\/jats:styled-content>\n sb2 was further confirmed by western blot analysis, and five putative cleavage sites were functionally characterized. Expression of the extracellular region alone partially rescued the defects of\n \n M<\/jats:italic>\n <\/jats:styled-content>\n omsb2<\/jats:italic>\n in appressorium formation and virulence. The cytoplasmic region of\n MoM<\/jats:styled-content>\n sb2, although dispensable for appressorium formation, was more important for penetration and invasive growth. Interestingly, the\n \n M<\/jats:italic>\n <\/jats:styled-content>\n omsb2 cbp1<\/jats:italic>\n double mutant deleted of both mucin genes was blocked in\n P<\/jats:styled-content>\n mk1 activation. It failed to form appressoria on artificial surfaces and was non\u2010pathogenic. In addition, we showed that\n MoM<\/jats:styled-content>\n sb2 interacts with\n R<\/jats:styled-content>\n as2 but not with\n MoC<\/jats:styled-content>\n dc42 in co\u2010immunoprecipitation assays. Overall, results from this study indicated that the extracellular and cytoplasmic regions of\n MoM<\/jats:styled-content>\n sb2 have distinct functions in appressorium formation, penetration and invasive growth, and\n MoM<\/jats:styled-content>\n sb2 has overlapping functions with\n C<\/jats:styled-content>\n bp1 in recognizing environmental signals for\n P<\/jats:styled-content>\n mk1 activation.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12847","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T15:58:14Z","timestamp":1427212694000},"page":"2969-2981","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Activation of the signalling mucin\n MoM<\/scp>\n sb2 and its functional relationship with\n C<\/scp>\n bp1 in\n \n M<\/i>\n <\/scp>\n agnaporthe oryzae<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Guanghui","family":"Wang","sequence":"first","affiliation":[{"name":"State 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Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Rong","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 China"},{"name":"Department of Botany and Plant Pathology Purdue University West Lafayette IN 47907 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