{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T01:42:13Z","timestamp":1785721333698,"version":"3.56.0"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T00:00:00Z","timestamp":1431907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Austrian Science Fund","award":["P21625-B20"],"award-info":[{"award-number":["P21625-B20"]}]},{"name":"EU-FP7 project AQUAVALENS","award":["311 846"],"award-info":[{"award-number":["311 846"]}]},{"name":"Austrian Federal Ministry"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Summary<\/jats:title>\n \n In order to elucidate the main predictors of\n \n V<\/jats:italic>\n <\/jats:styled-content>\n ibrio cholerae<\/jats:italic>\n dynamics and to estimate the risk of\n \n V<\/jats:italic>\n <\/jats:styled-content>\n ibrio cholera<\/jats:italic>\n \u2010related diseases, a recently developed direct detection approach based on fluorescence\n in situ<\/jats:italic>\n hybridization and solid\u2010phase cytometry (\n CARD\u2010FISH<\/jats:styled-content>\n \/\n SPC<\/jats:styled-content>\n ) was applied in comparison to cultivation for water samples from the lake Neusiedler See,\n A<\/jats:styled-content>\n ustria and three shallow alkaline lakes over a period of 20 months.\n \n V<\/jats:italic>\n <\/jats:styled-content>\n ibrio cholerae<\/jats:italic>\n attached to crustacean zooplankton was quantified via\n FISH<\/jats:styled-content>\n and epifluorescence microscopy. Concentrations obtained by\n CARD\u2010FISH<\/jats:styled-content>\n \/\n SPC<\/jats:styled-content>\n were significantly higher than those obtained by culture in 2011, but were mostly of similar magnitude in 2012. Maximum cell numbers were 1.26\u2009\u00d7\u200910\n 6<\/jats:sup>\n \n V<\/jats:italic>\n <\/jats:styled-content>\n . cholerae<\/jats:italic>\n per L in Neusiedler See and 7.59\u2009\u00d7\u200910\n 7<\/jats:sup>\n \n V<\/jats:italic>\n <\/jats:styled-content>\n . cholerae<\/jats:italic>\n per L in the shallow alkaline lakes. Only on a few occasions during summer was the crustacean zooplankton the preferred habitat for\n \n V<\/jats:italic>\n <\/jats:styled-content>\n . cholerae<\/jats:italic>\n . In winter,\n \n V<\/jats:italic>\n <\/jats:styled-content>\n . cholerae<\/jats:italic>\n was not culturable but could be quantified at all sites with\n CARD\u2010FISH<\/jats:styled-content>\n \/\n SPC<\/jats:styled-content>\n . Beside temperature, suspended solids, zooplankton and ammonium were the main predictors of\n \n V<\/jats:italic>\n <\/jats:styled-content>\n . cholerae<\/jats:italic>\n abundance in Neusiedler See, while in the shallow alkaline lakes it was organic carbon, conductivity and phosphorus. Based on the obtained concentrations a first estimation of the health risk for visitors of the lake could be performed.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12861","type":"journal-article","created":{"date-parts":[[2015,4,2]],"date-time":"2015-04-02T18:07:33Z","timestamp":1427998053000},"page":"4366-4378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Dynamics of\n \n V<\/i>\n <\/scp>\n ibrio cholerae<\/i>\n abundance in\n A<\/scp>\n ustrian saline lakes, assessed with quantitative solid\u2010phase cytometry"],"prefix":"10.1111","volume":"17","author":[{"given":"Sonja","family":"Schauer","sequence":"first","affiliation":[{"name":"Institute for Hygiene and Applied Immunology Medical University Vienna Vienna 1090 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Jakwerth","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Applied Immunology Medical 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