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It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two\u2010component system was shown to be required for virulence of several zoo\u2010pathogens, but it has never been investigated in phyto\u2010pathogens. In this paper, we investigate the role of the\n CpxAR<\/jats:styled-content>\n two\u2010component system\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n in\n \n D<\/jats:italic>\n <\/jats:styled-content>\n ickeya dadantii<\/jats:italic>\n , an enterobacterial phytopathogen that causes soft\u2010rot disease in a large variety of plant species.\n \n cpxA<\/jats:italic>\n <\/jats:styled-content>\n null mutant displays a constitutively phosphorylated\n CpxR<\/jats:styled-content>\n phenotype as shown by direct analysis of phosphorylation of\n CpxR<\/jats:styled-content>\n by a Phos\u2010Tag retardation gel approach. Virulence in plants is completely abolished in\n \n cpxA<\/jats:italic>\n <\/jats:styled-content>\n or\n \n cpxR<\/jats:italic>\n <\/jats:styled-content>\n mutants of\n \n D<\/jats:italic>\n <\/jats:styled-content>\n .\u2009dadantii<\/jats:italic>\n .\n \n I<\/jats:italic>\n <\/jats:styled-content>\n n planta<\/jats:italic>\n ,\n CpxAR<\/jats:styled-content>\n is only activated at an early stage of the infection process as shown by Phos\u2010Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of\n CpxAR<\/jats:styled-content>\n phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12874","type":"journal-article","created":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T07:52:33Z","timestamp":1428565953000},"page":"4415-4428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["The two\u2010component system\n CpxAR<\/scp>\n is essential for virulence in the phytopathogen bacteria\n \n D<\/i>\n <\/scp>\n ickeya dadantii<\/i>\n \u2005\n EC<\/scp>\n 3937"],"prefix":"10.1111","volume":"17","author":[{"given":"S\u00e9bastien","family":"Bontemps\u2010Gallo","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle UMR CNRS 8576 Universit\u00e9 des Sciences et Technologies de Lille Universit\u00e9 de Lille Villeneuve d'Ascq F\u201059655 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwige","family":"Madec","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle UMR CNRS 8576 Universit\u00e9 des Sciences et Technologies de Lille Universit\u00e9 de Lille Villeneuve d'Ascq F\u201059655 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Marie","family":"Lacroix","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle UMR CNRS 8576 Universit\u00e9 des Sciences et Technologies de Lille Universit\u00e9 de Lille Villeneuve d'Ascq F\u201059655 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02593-13"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.02.004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.32.090194.001221"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.16.5723-5731.2005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.3.595-600.2004"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12054"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.074484-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00143-10"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.24.090186.002123"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00218-07"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)35157-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05539.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn180"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2338.1982.tb01951.x"},{"key":"e_1_2_7_17_1","unstructured":"EPPO(1988)A1 and A2 Lists of Quarantine Pests. 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