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Microbial communities can degrade\n                    <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    and\n                    <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    , but little is known about their membership and metabolic potential. Microbial\u2010based remediation strategies will benefit from an ecological understanding of organisms involved in the breakdown of\n                    <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    and\n                    <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    into sulfur, carbon and nitrogen compounds. We performed metagenomic analysis of samples from two laboratory\u2010scale bioreactors used to study\n                    <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    and\n                    <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    degradation. Community analysis revealed the dominance of\n                    <jats:italic>Thiobacillus<\/jats:italic>\n                    spp., whose genomes harbour a previously unreported operon for\n                    <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    degradation. Genome\u2010based metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed but using energy gained from oxidation of sulfur compounds produced during\n                    <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content>\n                    <jats:sup>\u2212<\/jats:sup>\n                    degradation. Heterotrophs, including a bacterium from a previously uncharacterized phylum, compose a smaller portion of the reactor community. Predation by phage and eukaryotes is predicted to affect community dynamics. Genes for ammonium oxidation and denitrification were detected, indicating the potential for nitrogen removal, as required for complete remediation of wastewater. These findings suggest optimization strategies for reactor design, such as improved aerobic\/anaerobic partitioning and elimination of organic carbon from reactor feed.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12936","type":"journal-article","created":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T22:25:07Z","timestamp":1433197507000},"page":"4929-4941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":114,"title":["Bioreactor microbial ecosystems for thiocyanate and cyanide degradation unravelled with genome\u2010resolved metagenomics"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5402-8979","authenticated-orcid":false,"given":"Rose S.","family":"Kantor","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology University of California  Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Wynand","family":"van Zyl","sequence":"additional","affiliation":[{"name":"Center for Bioprocess Engineering Research Department of Chemical Engineering University of Cape Town  Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"van Hille","sequence":"additional","affiliation":[{"name":"Center for Bioprocess Engineering Research Department of Chemical Engineering University of Cape Town  Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian C.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Earth and Planetary Sciences University of California  Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan T. 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