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With this study, we showed the widespread presence of high\u2010level antibiotic resistant isolates on a collection of non\u2010susceptible Gram\u2010negative bacteria (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009232) recovered from soils. Bacteria were selected using amoxicillin, cefotaxime and imipenem, from sites representing different agricultural practices (extensive, intensive and organic). Striking levels of non\u2010susceptibility were noticed in intensive soils for norfloxacin (74%), streptomycin (50.7%) and tetracycline (46.6%); indeed, the exposure to intensive agricultural practices constituted a risk factor for non\u2010susceptibility to many antibiotics, multidrug resistance and production of extended\u2010spectrum \u03b2\u2010lactamases (\n                    <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>\n                    ). Analyses of non\u2010susceptibility highlighted that environmental and clinical bacteria from the same species might not share the same intrinsic resistance patterns, raising concerns for therapy choices in environment\u2010borne infections. The multiple sequence\u2010type IncI1\u2010driven spread of penicillinases (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>TEM\u20101<\/jats:sub>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>TEM\u2010135<\/jats:sub>\n                    <\/jats:styled-content>\n                    ),\n                    <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>SHV\u201012<\/jats:sub>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M\u20101<\/jats:sub>\n                    <\/jats:styled-content>\n                    ) and plasmid\u2010mediated AmpC \u03b2\u2010lactamases (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CMY\u20102<\/jats:sub>\n                    <\/jats:styled-content>\n                    ), produced by isolates that share their molecular features with isolates from humans and animals, suggests contamination of agricultural soils. This is also the first appearance of IncI1\/ST28\u2010harbouring\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M\u20101<\/jats:sub>\n                    <\/jats:styled-content>\n                    , which should be monitored to prevent their establishment as successfully dispersed plasmids. This research may help disclose paths of contamination by mobile antibiotic resistance determinants and the risks for their dissemination.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13021","type":"journal-article","created":{"date-parts":[[2015,8,17]],"date-time":"2015-08-17T04:17:04Z","timestamp":1439785024000},"page":"260-272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Influence of agricultural practice on mobile\n                    <scp>\n                      <i>bla<\/i>\n                    <\/scp>\n                    genes:\n                    <scp>IncI1<\/scp>\n                    \u2010bearing\n                    <scp>CTX<\/scp>\n                    \u2010\n                    <scp>M<\/scp>\n                    ,\n                    <scp>SHV<\/scp>\n                    ,\n                    <scp>CMY<\/scp>\n                    and\n                    <scp>TEM<\/scp>\n                    in\n                    <scp>\n                      <i>E<\/i>\n                    <\/scp>\n                    <i>scherichia coli<\/i>\n                    from intensive farming soils"],"prefix":"10.1111","volume":"18","author":[{"given":"Daniela","family":"Jones\u2010Dias","sequence":"first","affiliation":[{"name":"National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL\u2010AR\/HAI) Department of Infectious Diseases National Institute of Health Dr. Ricardo Jorge  Lisbon Portugal"},{"name":"Centre for the Studies of Animal Science Institute of Agrarian and Agri\u2010Food Sciences and Technologies Oporto University  Oporto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Manageiro","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL\u2010AR\/HAI) Department of Infectious Diseases National Institute of Health Dr. Ricardo Jorge  Lisbon Portugal"},{"name":"Centre for the Studies of Animal Science Institute of Agrarian and Agri\u2010Food Sciences and Technologies Oporto University  Oporto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Cani\u00e7a","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL\u2010AR\/HAI) Department of Infectious Diseases National Institute of Health Dr. Ricardo Jorge  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-13-114"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2015.02.023"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3439"},{"key":"e_1_2_6_5_1","unstructured":"Bonnet R. 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