{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:43:52Z","timestamp":1792813432558,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T00:00:00Z","timestamp":1444780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"EU FP7 project SysteMTb"},{"name":"Intramural Research Program of NIAID"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    An organism's metabolic activity leaves an extracellular footprint and dynamic changes in this exometabolome inform about nutrient uptake, waste disposal and signalling activities. Using non\u2010targeted mass spectrometry, we report exometabolome dynamics of hypoxia\u2010induced, non\u2010replicating mycobacteria that are thought to play a role in latent tuberculosis. Despite evidence of active metabolism, little is known about the mechanisms enabling obligate aerobic mycobacteria to cope with hypoxia, resulting in long\u2010term survival and increased chemotherapeutic tolerance. The dynamics of 379 extracellular compounds of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>ycobacterium smegmatis<\/jats:italic>\n                    were deconvoluted with a genome\u2010scale metabolic reaction\u2010pair network to generate hypotheses about intracellular pathway usage. Time\u2010resolved\n                    <jats:sup>13<\/jats:sup>\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    \u2010tracing and mutant experiments then demonstrated a crucial, energy\u2010generating role of asparagine utilization and non\u2010generic usage of the glyoxylate shunt for hypoxic fitness. Experiments with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009bovis<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009tuberculosis<\/jats:italic>\n                    revealed the general relevance of asparagine fermentation and a variable contribution of the glyoxylate shunt to non\u2010replicative, hypoxic survival between the three species.\n                  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