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We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    cells ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Super\u2010resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high\u2010throughput limited dilution approach and successfully recovered a\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>exillifera<\/jats:italic>\n                    sp., strain harbouring chlamydiae (93% 16\n                    <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\n                    r\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    sequence identity to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>imkania negevensis<\/jats:italic>\n                    ), tentatively named \u2018\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>andidatus<\/jats:italic>\n                    \u2005\n                    <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\n                    eptunochlamydia vexilliferae\u2019. Transmission electron microscopy in combination with fluorescence\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13111","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T22:35:03Z","timestamp":1446590103000},"page":"2405-2417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Chlamydial seasonal dynamics and isolation of \u2018\n                    <scp>\n                      <i>C<\/i>\n                    <\/scp>\n                    <i>andidatus<\/i>\n                    \u2005\n                    <scp>N<\/scp>\n                    eptunochlamydia vexilliferae\u2019 from a\n                    <scp>T<\/scp>\n                    yrrhenian coastal lake"],"prefix":"10.1111","volume":"18","author":[{"given":"Ilaria","family":"Pizzetti","sequence":"first","affiliation":[{"name":"Water Research Institute IRSA\u2010CNR  via Salaria km 29,300, 00015 Monterotondo Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederik","family":"Schulz","sequence":"additional","affiliation":[{"name":"University of Vienna, Department of Microbiology and Ecosystem Science  Althanstrasse 14 A\u20101090 Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1\u0161","family":"Tyml","sequence":"additional","affiliation":[{"name":"University of South Bohemia, Faculty of Science  Brani\u0161ovsk\u00e1 31 370 05 \u010cesk\u00e9 Bud\u011bjovice Czech Republic"},{"name":"Masaryk University, Department of Botany and Zoology, Faculty of Science  Kotl\u00e1\u0159sk\u00e1 2 61137 Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard M.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Marine Microbiology  Celsiusstrasse 1 D\u201028359 Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Amann","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Marine Microbiology  Celsiusstrasse 1 D\u201028359 Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Horn","sequence":"additional","affiliation":[{"name":"University of Vienna, Department of Microbiology and Ecosystem Science  Althanstrasse 14 A\u20101090 Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Fazi","sequence":"additional","affiliation":[{"name":"Water Research Institute IRSA\u2010CNR  via Salaria km 29,300, 00015 Monterotondo Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg427"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00225.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.1.115-121.1997"},{"key":"e_1_2_6_5_1","unstructured":"APAT andIRSA\u2010CNR(2003)Metodi analitici per le acque. 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