{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T13:37:17Z","timestamp":1768484237017,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T00:00:00Z","timestamp":1453075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FP7 Marie Curie Actions of the European Commission","award":["FP7-PEOPLE-2012-ITN-316472"],"award-info":[{"award-number":["FP7-PEOPLE-2012-ITN-316472"]}]},{"name":"Flemish Fund for Scientific Research","award":["FWO15\/PDOH1\/084"],"award-info":[{"award-number":["FWO15\/PDOH1\/084"]}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Summary<\/jats:title>\n \n \n B<\/jats:italic>\n <\/jats:styled-content>\n acillus vireti<\/jats:italic>\n is a nitrate\u2010ammonifying bacterium and a partial denitrifier, reducing\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n ,\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n ,\n NO<\/jats:styled-content>\n and\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n with\n NarG<\/jats:styled-content>\n ,\n NrfA<\/jats:styled-content>\n ,\n CbaA<\/jats:styled-content>\n and\n NosZ<\/jats:styled-content>\n respectively. Growth is optimized through successive use of the electron acceptors\n \n \n O\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n and\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n , followed by\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n ,\n NO<\/jats:styled-content>\n and\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n . Fermentation takes place simultaneously with anaerobic respiration. When grown in batch culture with 5\u2009mM initial\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n , transcription of\n \n nrfA<\/jats:italic>\n <\/jats:styled-content>\n was high and most\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n was reduced to\n \n \n NH\n 4<\/jats:sub>\n +<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n . With 20\u2009mM initial\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n ,\n \n nrfA<\/jats:italic>\n <\/jats:styled-content>\n transcription was lower and more than 50% of the nitrate was recovered as\n NO<\/jats:styled-content>\n ,\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n and\n \n \n N\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n . Analysis of gene transcription patterns and corresponding gas kinetics indicated that\n \n \n O\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n and\n \n \n NO\n 2<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n or\n NO<\/jats:styled-content>\n are main controllers of\n \n nrfA<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n nirB<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n cbaA<\/jats:italic>\n <\/jats:styled-content>\n and\n \n nosZ<\/jats:italic>\n <\/jats:styled-content>\n transcription. This was corroborated by analyses of putative binding regions for specific transcriptional regulators. Furthermore, we demonstrate that\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n reduction in\n \n B<\/jats:italic>\n <\/jats:styled-content>\n .\u2009vireti<\/jats:italic>\n supports growth. The high\n \n nosZ<\/jats:italic>\n <\/jats:styled-content>\n transcription but low\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n production seen at 5\u2009mM\n \n \n NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n <\/jats:roman>\n <\/jats:styled-content>\n implies that this organism can use\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n reductase to scavenge\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n from other organisms in the soil, thus possibly acting as a net sink for\n \n \n N\n 2<\/jats:sub>\n O\n <\/jats:roman>\n <\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/1462-2920.13124","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T01:25:02Z","timestamp":1447032302000},"page":"2937-2950","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Regulation of nitrogen metabolism in the nitrate\u2010ammonifying soil bacterium\n \n B<\/i>\n <\/scp>\n acillus vireti<\/i>\n and evidence for its ability to grow using\n \n \n N\n 2<\/sub>\n O\n <\/scp>\n <\/scp>\n as electron acceptor"],"prefix":"10.1111","volume":"18","author":[{"given":"Daniel","family":"Mania","sequence":"first","affiliation":[{"name":"Department of Chemistry, Biotechnology and Food Science Norwegian University of Life Science \u00c5s Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Heylen","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Department of Biochemistry and Microbiology University of Ghent Gent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob J. 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