{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:56:10Z","timestamp":1790232970460,"version":"4.1.0"},"reference-count":87,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T00:00:00Z","timestamp":1464134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Infections due to multidrug\u2010resistant bacteria represent a major global health challenge. To combat this problem, new antibiotics are urgently needed and some plant\u2010associated bacteria are a promising source. The rhizobacterium\n                    <jats:italic>Serratia plymuthica<\/jats:italic>\n                    A153 produces several bioactive secondary metabolites, including the anti\u2010oomycete and antifungal haterumalide, oocydin A and the broad spectrum polyamine antibiotic, zeamine. In this study, we show that A153 produces a second broad spectrum antibiotic, andrimid. Using genome sequencing, comparative genomics and mutagenesis, we defined new genes involved in andrimid (adm) biosynthesis. Both the expression of the\n                    <jats:italic>adm<\/jats:italic>\n                    gene cluster and regulation of andrimid synthesis were investigated. The biosynthetic cluster is operonic and its expression is modulated by various environmental cues, including temperature and carbon source. Analysis of the genome context of the\n                    <jats:italic>adm<\/jats:italic>\n                    operon revealed a gene encoding a predicted LysR\u2010type regulator, AdmX, apparently unique to\n                    <jats:italic>Serratia<\/jats:italic>\n                    strains. Mutagenesis and gene expression assays demonstrated that AdmX is a transcriptional activator of the\n                    <jats:italic>adm<\/jats:italic>\n                    gene cluster. At the post\u2010transcriptional level, the expression of the\n                    <jats:italic>adm<\/jats:italic>\n                    cluster is positively regulated by the RNA chaperone, Hfq, in an RpoS\u2010independent manner. Our results highlight the complexity of andrimid biosynthesis \u2013 an antibiotic with potential clinical and agricultural utility.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13241","type":"journal-article","created":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T22:16:52Z","timestamp":1456438612000},"page":"3635-3650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Biosynthesis of the acetyl\u2010CoA carboxylase\u2010inhibiting antibiotic, andrimid in\n                    <i>Serratia<\/i>\n                    is regulated by Hfq and the LysR\u2010type transcriptional regulator, AdmX"],"prefix":"10.1111","volume":"18","author":[{"given":"Miguel A.","family":"Matilla","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Cambridge Tennis Court Road Cambridge CB2 1QW UK"},{"name":"Department of Environmental Protection Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas Prof. Albareda 1 Granada 18008 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Nogellova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge Tennis Court Road Cambridge CB2 1QW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Morel","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas Prof. Albareda 1 Granada 18008 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tino","family":"Krell","sequence":"additional","affiliation":[{"name":"Department of Environmental Protection Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas Prof. Albareda 1 Granada 18008 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George P. C.","family":"Salmond","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge Tennis Court Road Cambridge CB2 1QW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0434.2001.00585.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02202093"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2009.01926.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.7.3328-3338.2002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3380"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04939.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00052-06"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00780"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1128"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.23.4636"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.09.007"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07673.x"},{"key":"e_1_2_6_14_1","first-page":"1","article-title":"Using Serratia plymuthica to control fungal pathogens of plants","volume":"2","author":"Vleesschauwer D.","year":"2007","journal-title":"CAB Rev Perspect Agric Vet Sci Nutr Nat Resour"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR12.051"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.09.090"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176667"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh458"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00248a055"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402989200"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06068"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00008"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3128-3"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1129"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420009675.ch10"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00137"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12198"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.11.6557-6567.1990"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200806121"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008681608400"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00667-15"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja063194c"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402404"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90599-7"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-94"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3975"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039538"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00054-12"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806873105"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-26"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8531-8536.2005"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00017-11"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162918"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1365-z"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/022772-0"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb800085g"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00040-13"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.401026"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01546-14"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12839"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00373-16"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12028"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07043.x"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00231"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2008.00635.x"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00087a020"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1039\/b817069j"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/np068054v"},{"key":"e_1_2_6_59_1","first-page":"7","article-title":"Anti\u2010Bacillus substance in the marine sponge, Hyatella species, produced by an associated Vibrio species bacterium","volume":"78","author":"Oclarit J.M.","year":"1994","journal-title":"Microbios"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.08.011"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00087-12"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-008-9568-6"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.827"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja2030728"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02794.x"},{"key":"e_1_2_6_67_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2010.78"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-013-1338-9"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1039\/b801747f"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.50.270"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931429100"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-145-12-3557"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0765.2009.00390.x"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0038-0717(01)00109-2"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700962104"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420005585.ch1"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2615"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1401734111"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.3390\/md8122946"},{"key":"e_1_2_6_81_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2011.00259.x"},{"key":"e_1_2_6_82_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.055780-0"},{"key":"e_1_2_6_83_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02532.x"},{"key":"e_1_2_6_84_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1531"},{"key":"e_1_2_6_85_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.9.3469"},{"key":"e_1_2_6_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00268.x"},{"key":"e_1_2_6_87_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.090092"},{"key":"e_1_2_6_88_1","doi-asserted-by":"publisher","DOI":"10.2741\/e457"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1462-2920.13241","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1462-2920.13241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.13241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T21:43:50Z","timestamp":1761860630000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.13241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,25]]},"references-count":87,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/1462-2920.13241"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.13241","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,25]]},"assertion":[{"value":"2015-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}