{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:04:53Z","timestamp":1789664693864,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T00:00:00Z","timestamp":1461974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Dehalobacter sp.<\/jats:italic>\n                    strain UNSWDHB can dechlorinate up to 4 mM trichloromethane at a rate of 0.1 mM per day to dichloromethane and 1,1,2\u2010trichloroethane (1 mM, 0.1 mM per day) with the unprecedented product profile of 1,2\u2010dichloroethane and vinyl chloride. 1,1,1\u2010trichloroethane and 1,1\u2010dichloroethane were slowly utilized by strain UNSWDHB and were not completely removed, with minimum threshold concentrations of 0.12 mM and 0.07 mM respectively under growth conditions. Enzyme kinetic experiments confirmed strong substrate affinity for trichloromethane and 1,1,2\u2010trichloroethane (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    \u2009=\u200930 and 62 \u00b5M respectively) and poor substrate affinity for 1,1,1\u2010trichloroethane and 1,1\u2010dichloroethane (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    \u2009=\u2009238 and 837 \u00b5M respectively). Comparison of enzyme kinetic and growth data with other trichloromethane respiring organisms (\n                    <jats:italic>Dehalobacter sp.<\/jats:italic>\n                    strain CF and\n                    <jats:italic>Desulfitobacterium sp.<\/jats:italic>\n                    strain PR) suggests an adaptation of strain UNSWDHB to trichloromethane. The trichloromethane RDase (TmrA) expressed by strain UNSWDHB was identified by BN\u2010PAGE and functionally characterized. Amino acid comparison of homologous RDases from all three organisms revealed only six significant amino acid substitutions\/deletions, which are likely to be crucial for substrate specificity. Furthermore, strain UNSWDHB was shown to grow without exogenous supply of cobalamin confirming genomic\u2010based predictions of a fully functional cobalamin synthetic pathway.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13287","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T14:12:21Z","timestamp":1457791941000},"page":"3092-3105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Isolation and characterization of\n                    <i>Dehalobacter sp.<\/i>\n                    strain UNSWDHB capable of chloroform and chlorinated ethane respiration"],"prefix":"10.1111","volume":"18","author":[{"given":"Yie K.","family":"Wong","sequence":"first","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie I.","family":"Holland","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haluk","family":"Ertan","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney Australia"},{"name":"Department of Molecular Biology and Genetics Istanbul University  Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Manefield","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,30]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"ASTDR(2015a)Toxological profile for chloroform[WWW document]. 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