{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T13:00:00Z","timestamp":1768482000461,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T00:00:00Z","timestamp":1465344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Clarifying glycosyltrasferases (GTs) function is of significance for the development of GT inhibitors as drugs, and the use of GTs to glycodiversify small molecules in the search of drug leads. While many\n                    <jats:italic>Actinomyces<\/jats:italic>\n                    natural\u2010product GTs had been functionally characterized, our understanding towards\n                    <jats:italic>Bacillus<\/jats:italic>\n                    natural\u2010product GTs is so far very limited. Herein, genome\u2010wide identification of macrolide GT genes from marine\u2010derived\n                    <jats:italic>Bacillus methylotrophicus<\/jats:italic>\n                    B\u20109987 revealed the presence of three macrolide GT genes\n                    <jats:italic>bmmGT1<\/jats:italic>\n                    \u22123. While\n                    <jats:italic>bmmGT1<\/jats:italic>\n                    was previously revealed to be involved in the biosynthesis of\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010acyltransferase (AT) polyketides compounds macrolactins (MLNs) and bacillaenes (BAEs), the functions of\n                    <jats:italic>bmmGT2<\/jats:italic>\n                    and\n                    <jats:italic>bmmGT3<\/jats:italic>\n                    were probed, demonstrating that they are capable to biochemically catalyze glycosylation of MLNs and BAEs as well but interestingly with different regioselectivity, affording four new MLNs analogs. Notably, further genome mining revealed that the orthologs of these three macrolide GT genes showed a regular distribution in the\n                    <jats:italic>subtilis<\/jats:italic>\n                    \u2010 and the\n                    <jats:italic>cereus<\/jats:italic>\n                    \u2010clade\n                    <jats:italic>Bacillus<\/jats:italic>\n                    strains; interestingly,\n                    <jats:italic>bmmGT1<\/jats:italic>\n                    orthologs only occurred in the\n                    <jats:italic>subtilis<\/jats:italic>\n                    \u2010clade\n                    <jats:italic>Bacillus<\/jats:italic>\n                    , and they were also found in the genomes of\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    strains, suggesting their close phylogenetic relationship. These results provide the first significant insight into the important roles of\n                    <jats:italic>Bacillus<\/jats:italic>\n                    macrolide GTs in the biology of the species.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13367","type":"journal-article","created":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T05:58:23Z","timestamp":1462082303000},"page":"4770-4781","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Genome\u2010wide identification and characterization of macrolide glycosyltransferases from a marine\u2010derived\n                    <i>Bacillus<\/i>\n                    strain and their phylogenetic distribution"],"prefix":"10.1111","volume":"18","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Qin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huayue","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Tian","sequence":"additional","affiliation":[{"name":"First Institute of Oceanography, State Oceanic Administration  6 Xianxialing Road Qingdao SD 266061 P. R. China"},{"name":"Qingdao University of Science &amp; Technology  53 Zhen Zhou Road Qingdao SD 266042 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenli","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China Qingdao 266003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.0802.116"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00018-14"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.048488-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607897104"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.023267-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610503104"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn663"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2011.04.013"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1325"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn879"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.02.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2009.05.019"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1np00045d"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-013-0164-0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00201a036"},{"key":"e_1_2_7_17_1","unstructured":"Ji Y.H. 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