{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:32:28Z","timestamp":1790404348035,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T00:00:00Z","timestamp":1522713600000},"content-version":"am","delay-in-days":328,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2017,5,10]],"date-time":"2017-05-10T00:00:00Z","timestamp":1494374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EAR\u20101529461"],"award-info":[{"award-number":["EAR\u20101529461"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The \u2018CH\n                    <jats:sub>4<\/jats:sub>\n                    oversaturation paradox\u2019 has been observed in oxygen\u2010rich marine and lake waters, and viewed to significantly contribute to biosphere cycling of methane, a potent greenhouse gas. Our study focused on the intriguing well\u2010defined pelagic methane enriched zone (PMEZ) in freshwater lakes. Spiking Yellowstone Lake PMEZ samples with\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010labeled potential methanogenesis substrates found only\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010methylphosphonate (MPn) resulted in\n                    <jats:sup>13<\/jats:sup>\n                    CH\n                    <jats:sub>4<\/jats:sub>\n                    generation. In 16S rRNA gene Illumina libraries, four\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. operational taxonomic units surprisingly accounted for \u223c11% abundance in the PMEZ community.\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. isolates were also obtained from MPn enrichments with PMEZ water; they were most aggressive in MPn metabolism and their 16S rRNA gene sequences matched 35% of the Illumina PMEZ\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    reads. Further, two key genes encoding C\u2010P lyase (\n                    <jats:italic>phnJL<\/jats:italic>\n                    , an important enzyme for dealkylation of MPn), were only amplifiable from PMEZ DNA and all PCR generated\n                    <jats:italic>phnJL<\/jats:italic>\n                    clones matched those of the\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. isolates. Notably, methanogen 16S rRNA signatures were absent in all Illumina libraries and\n                    <jats:italic>mcrA<\/jats:italic>\n                    was not detected via PCR. Collectively, these observations are consistent with the conclusion that MPn metabolism contributes significantly to CH\n                    <jats:sub>4<\/jats:sub>\n                    oversaturation in Yellowstone Lake and likely other oxic freshwater lake environments, and that\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. populations are critical participants.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13747","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T03:14:43Z","timestamp":1491362083000},"page":"2366-2378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["Methylphosphonate metabolism by\n                    <i>Pseudomonas<\/i>\n                    sp. populations contributes to the methane oversaturation paradox in an oxic freshwater lake"],"prefix":"10.1111","volume":"19","author":[{"given":"Qian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Land Resources and Environmental Sciences Montana State University Bozeman MT 59717 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Dore","sequence":"additional","affiliation":[{"name":"Department of Land Resources and Environmental Sciences Montana State University Bozeman MT 59717 USA"},{"name":"Montana Institute on Ecosystems, Montana State University Bozeman MT 59717 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R.","family":"McDermott","sequence":"additional","affiliation":[{"name":"Department of Land Resources and Environmental Sciences Montana State University Bozeman MT 59717 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1196808"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps088055"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6350"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2013.03.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1029\/JC086iC11p11029"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.122"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5346"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg500"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02466.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1244"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.5194\/bg-7-1099-2010"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16179"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1994.39.6.1298"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/lno.10335"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01714"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01585"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02643-15"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo639"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr381"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-8904(95)00319-3"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/es500987j"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110716108"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1029\/1999GB001209"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1500873112"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.56"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00210"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/368732a0"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo234"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1996.41.5.1052"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.164.3875.37"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(69)91639-9"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9320-4"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.5194\/bg-13-3163-2016"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-11-3521"},{"key":"e_1_2_8_37_1","first-page":"863","article-title":"Methanoarchaea 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