{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T17:54:00Z","timestamp":1789322040470,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,7,7]],"date-time":"2017-07-07T00:00:00Z","timestamp":1499385600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"publisher","award":["102 101 0025"],"award-info":[{"award-number":["102 101 0025"]}],"id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001381","name":"National Research Foundation Singapore","doi-asserted-by":"publisher","award":["NRF\u2010CRP5\u20102009\u201005"],"award-info":[{"award-number":["NRF\u2010CRP5\u20102009\u201005"]}],"id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Polybrominated diphenyl ethers (PBDEs), chemicals commonly used as flame\u2010retardants in consumer products, are emerging persistent organic pollutants that are ubiquitous in the environment. In this study, we report a PBDE\u2010respiring isolate \u2013\n                    <jats:italic>Dehalococcoides mccartyi<\/jats:italic>\n                    strain GY50, which debrominates the most toxic tetra\u2010 and penta\u2010BDE congeners (\u223c1.4 \u00b5M) to diphenyl ether within 12 days with hydrogen as the electron donor. The complete genome sequence revealed 26 reductive dehalogenase homologous genes (\n                    <jats:italic>rdhA<\/jats:italic>\n                    s), among which three genes (\n                    <jats:italic>pbrA1<\/jats:italic>\n                    ,\n                    <jats:italic>pbrA2<\/jats:italic>\n                    and\n                    <jats:italic>pbrA3<\/jats:italic>\n                    ) were highly expressed during PBDE debromination. After 10 transfers of GY50 with trichloroethene or 2,4,6\u2010trichlorophenol as the electron acceptor instead of PBDEs, the\n                    <jats:italic>ssrA<\/jats:italic>\n                    \u2010specific genome island (\n                    <jats:italic>ssrA<\/jats:italic>\n                    \u2010GI) containing\n                    <jats:italic>pbrA1<\/jats:italic>\n                    and\n                    <jats:italic>pbrA2<\/jats:italic>\n                    was deleted from the genome of strain GY50, leading to two variants (strain GY52 with trichloroethene, strain GY55 with 2,4,6\u2010trichlorophenol) with identically impaired debromination capabilities (debromination of penta\u2010\/tetra\u2010BDEs ceased at di\u2010BDE 15). Through analysis of Illumina paired\u2010end sequencing data, we identified read pairs that probably came from variants that contain\n                    <jats:italic>ssrA<\/jats:italic>\n                    \u2010GI deletions, indicating their possible presence in the\u00a0original strain GY50 culture. The two variant strains provide real\u2010time examples on rapid evolution of organohalide\u2010respiring organisms. As PBDE\u2010respiring organisms, GY50\u2010like strains may serve as key players in detoxifying PBDEs in contaminated environments.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13817","type":"journal-article","created":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T07:30:59Z","timestamp":1497511859000},"page":"2906-2915","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Loss of the\n                    <i>ssrA<\/i>\n                    genome island led to partial debromination in the PBDE respiring\n                    <i>Dehalococcoides mccartyi<\/i>\n                    strain GY50"],"prefix":"10.1111","volume":"19","author":[{"given":"Chang","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering National University of Singapore Singapore 117576 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew 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