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This study aimed to understand the impact of uranium concentration on microbial communities, in order to identify and describe potential breakpoints in microbial ecosystem services. This is the first study to report\n                    <jats:italic>in situ<\/jats:italic>\n                    deployment of uranium\u2010spiked sediments along a concentration gradient (0\u20134000 mg U\u00a0kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), with the study design maximising the advantages of both field surveys and laboratory manipulative studies. Changes to microbial communities were characterised through the use of amplicon and shotgun metagenomic next\u2010generation sequencing. Significant changes to taxonomic and functional community assembly occurred at a concentration of 1500 mg U\u00a0kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    sediment and above. At uranium concentrations of\u2009\u2265\u20091500 mg U\u00a0kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    , genes associated with methanogenic consortia and processes increased in relative abundance, while numerous significant changes were also seen in the relative abundances of genes involved in nitrogen cycling. Such alterations in carbon and nitrogen cycling pathways suggest that taxonomic and functional changes to microbial communities may result in changes in ecosystem processes and resilience.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13839","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:12:09Z","timestamp":1497917529000},"page":"3323-3341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Effects of uranium concentration on microbial community structure and functional potential"],"prefix":"10.1111","volume":"19","author":[{"given":"Brodie","family":"Sutcliffe","sequence":"first","affiliation":[{"name":"Macquarie University Sydney New South Wales, 2109 Australia"},{"name":"Commonwealth Scientific and Industrial Research Organisation (CSIRO) Canberra Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A.","family":"Chariton","sequence":"additional","affiliation":[{"name":"Macquarie University Sydney New South Wales, 2109 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Harford","sequence":"additional","affiliation":[{"name":"Supervising Scientist Branch, Department of the Environment and Energy Darwin Northern Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant C.","family":"Hose","sequence":"additional","affiliation":[{"name":"Macquarie University Sydney New South Wales, 2109 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Greenfield","sequence":"additional","affiliation":[{"name":"Commonwealth Scientific and Industrial Research Organisation (CSIRO) Canberra Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam D. 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