{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:26:36Z","timestamp":1790702796868,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T00:00:00Z","timestamp":1507852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Chemours Company"},{"name":"Strategic Environmental Research and Development Program (SERDP)"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The microbial mixed culture RM grows with dichloromethane (DCM) as the sole energy source generating acetate, methane, chloride and biomass as products. Chloromethane (CM) was not an intermediate during DCM utilization consistent with the observation that CM could not replace DCM as a growth substrate. Interestingly, cultures that received DCM and CM together degraded both compounds concomitantly. Transient hydrogen (H\n                    <jats:sub>2<\/jats:sub>\n                    ) formation reaching a maximum concentration of 205\u2009\u00b1\u200913 ppmv was observed in cultures growing with DCM, and the addition of exogenous H\n                    <jats:sub>2<\/jats:sub>\n                    at concentrations exceeding 3000 ppmv impeded DCM degradation. In contrast, CM degradation in culture RM had a strict requirement for H\n                    <jats:sub>2<\/jats:sub>\n                    . Following five consecutive transfers on CM and H\n                    <jats:sub>2<\/jats:sub>\n                    ,\n                    <jats:italic>Acetobacterium<\/jats:italic>\n                    16S rRNA gene sequences dominated the culture and the DCM\u2010degrader\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Dichloromethanomonas elyunquensis was eliminated, consistent with the observation that the culture lost the ability to degrade DCM. These findings demonstrate that culture RM harbours different populations responsible for anaerobic DCM and CM metabolism, and further imply that the DCM and CM degradation pathways are mechanistically distinct. H\n                    <jats:sub>2<\/jats:sub>\n                    generated during DCM degradation is consumed by the hydrogenotrophic CM degrader, or may fuel other hydrogenotrophic processes, including organohalide respiration, methanogenesis and H\n                    <jats:sub>2<\/jats:sub>\n                    \/CO\n                    <jats:sub>2<\/jats:sub>\n                    reductive acetogenesis.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.13945","type":"journal-article","created":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T03:52:36Z","timestamp":1506916356000},"page":"4784-4796","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Mutualistic interaction between dichloromethane\u2010 and chloromethane\u2010degrading bacteria in an anaerobic mixed culture"],"prefix":"10.1111","volume":"19","author":[{"given":"Gao","family":"Chen","sequence":"first","affiliation":[{"name":"Center for Environmental Biotechnology University of Tennessee Knoxville TN 37996 USA"},{"name":"Department of Civil and Environmental Engineering University of Tennessee Knoxville TN 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Kleindienst","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology University of Tennessee Knoxville TN 37996 USA"},{"name":"Oak Ridge National Laboratory University of Tennessee and Oak Ridge National Laboratory (UT\u2010ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division Oak Ridge TN 37831 USA"},{"name":"Center for Applied Geosciences Department of Geosciences, Eberhard Karls University T\u00fcbingen T\u00fcbingen 72074 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Parsons Denver CO 80203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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DuPont de Nemours and Company Wilmington DE 19805 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward S.","family":"Seger","sequence":"additional","affiliation":[{"name":"The Chemours Company Wilmington DE 19899 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology University of Tennessee Knoxville TN 37996 USA"},{"name":"Department of Civil and Environmental Engineering University of Tennessee Knoxville TN 37996 USA"},{"name":"Oak Ridge National Laboratory University of Tennessee and Oak Ridge National Laboratory (UT\u2010ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division Oak Ridge TN 37831 USA"},{"name":"Department of Microbiology University of Tennessee Knoxville TN 37996 USA"},{"name":"Department of Biosystems Engineering and Soil Science University of Tennessee Knoxville TN 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es061438n"},{"key":"e_1_2_7_3_1","unstructured":"ATSDR(1998)Toxicological profile for chloromethane. 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