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We found that\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    manipulates its host to increase nutrients in tomato xylem sap, enabling it to grow better in sap from infected plants than in sap from healthy plants. Untargeted GC\/MS metabolomics identified 22 metabolites enriched in\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    \u2010infected sap. Eight of these could serve as sole carbon or nitrogen sources for\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    . Putrescine, a polyamine that is not a sole carbon or nitrogen source for\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    , was enriched 76\u2010fold to 37 \u00b5M in\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    \u2010infected sap.\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    synthesized putrescine via a SpeC ornithine decarboxylase. A \u0394\n                    <jats:italic>speC<\/jats:italic>\n                    mutant required\u2009\u2265\u200915 \u00b5M exogenous putrescine to grow and could not grow alone in xylem even when plants were treated with putrescine. However, co\u2010inoculation with wildtype rescued\n                    <jats:italic>\u0394speC<\/jats:italic>\n                    growth, indicating\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    produced and exported putrescine to xylem sap. Intriguingly, treating plants with putrescine before inoculation accelerated wilt symptom development and\n                    <jats:italic>R. solanacearum<\/jats:italic>\n                    growth and systemic spread. Xylem putrescine concentration was unchanged in putrescine\u2010treated plants, so the exogenous putrescine likely accelerated disease indirectly by affecting host physiology. These results indicate that putrescine is a pathogen\u2010produced virulence metabolite.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14020","type":"journal-article","created":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T05:23:12Z","timestamp":1512624192000},"page":"1330-1349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":167,"title":["Metabolomics of tomato xylem sap during bacterial wilt reveals\n                    <i>Ralstonia solanacearum<\/i>\n                    produces abundant putrescine, a metabolite that accelerates wilt disease"],"prefix":"10.1111","volume":"20","author":[{"given":"Tiffany M.","family":"Lowe\u2010Power","sequence":"first","affiliation":[{"name":"Department of Plant Pathology University of Wisconsin \u2013 Madison Madison WI 53706 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor 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