{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:11:53Z","timestamp":1777504313120,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T00:00:00Z","timestamp":1535587200000},"content-version":"vor","delay-in-days":60,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Desulfitobacterium hafniense<\/jats:italic>\n                    Y51 has been widely used in investigations of perchloroethylene (PCE) biodegradation, but limited information exists on its other physiological capabilities. We investigated how\n                    <jats:italic>D. hafniense<\/jats:italic>\n                    Y51 confronts the debilitating limitations of not having enough electron donor (lactate), or electron acceptor (fumarate) during cultivation in chemostats. The residual concentrations of the substrates supplied in excess were much lower than expected. Transcriptomics, proteomics and fluxomics were integrated to investigate how this phenomenon was regulated. Through diverse regulation at both transcriptional and translational levels, strain Y51 turned to fermenting the excess lactate and disproportionating the excess fumarate under fumarate\u2010 and lactate\u2010limiting conditions respectively. Genes and proteins related to the utilization of a variety of alternative electron donors and acceptors absent from the medium were induced, apparently involving the Wood\u2013Ljungdahl pathway. Through this metabolic flexibility,\n                    <jats:italic>D. hafniense<\/jats:italic>\n                    Y51 may be able to switch between different metabolic capabilities under limiting conditions.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14295","type":"journal-article","created":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T18:41:46Z","timestamp":1529433706000},"page":"2652-2669","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Metabolic flexibility of a prospective bioremediator:\n                    <i>Desulfitobacterium hafniense<\/i>\n                    Y51 challenged in chemostats"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2946-1591","authenticated-orcid":false,"given":"Sviatlana","family":"Marozava","sequence":"first","affiliation":[{"name":"Institute of Groundwater Ecology Helmholtz Zentrum M\u00fcnchen  Ingolst\u00e4dter Landstra\u00dfe 1, 85764 Neuherberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Vargas\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Molecular Cell Physiology, Faculty of Science VU University Amsterdam  De Boelelaan 1085, 1081 HV Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Tian","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliane","family":"Merl\u2010Pham","sequence":"additional","affiliation":[{"name":"Core Facility Proteomics Helmholtz Zentrum M\u00fcnchen  Heidemannstra\u00dfe 1, 80939 M\u00fcnchen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Braster","sequence":"additional","affiliation":[{"name":"Molecular Cell Physiology, Faculty of Science VU University Amsterdam  De Boelelaan 1085, 1081 HV Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainer U.","family":"Meckenstock","sequence":"additional","affiliation":[{"name":"Institute of Groundwater Ecology Helmholtz Zentrum M\u00fcnchen  Ingolst\u00e4dter Landstra\u00dfe 1, 85764 Neuherberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hauke","family":"Smidt","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilfred F.M.","family":"R\u00f6ling","sequence":"additional","affiliation":[{"name":"Molecular Cell Physiology, Faculty of Science VU University Amsterdam  De Boelelaan 1085, 1081 HV Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans V.","family":"Westerhoff","sequence":"additional","affiliation":[{"name":"Molecular Cell Physiology, Faculty of Science VU University Amsterdam  De Boelelaan 1085, 1081 HV Amsterdam The Netherlands"},{"name":"Synthetic Systems Biology, SILS, University of Amsterdam  Amsterdam The Netherlands"},{"name":"Manchester Centre for Integrative Systems Biology School of Chemical Engineering and Analytical Science, University of Manchester  Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400266m"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/es051849o"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-1401-9_3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr800982s"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth347"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-347"},{"key":"e_1_2_6_9_1","first-page":"442","article-title":"Desulfitobacterium hafniense sp. nov., an anaerobic, reductively dechlorinating bacterium","volume":"46","author":"Christiansen N.","year":"1996","journal-title":"Int J Syst Evol Microbiol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1986.tb01748.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1473550411000012"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00731.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28939-0"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-005-0252-z"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.205336"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-1072-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27924-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02208-07"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-21"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.646-666.1998"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(74)90051-6"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fix135"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02370-14"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2014.02.005"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.5b00715"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201100549"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12433"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.6.2262-2274.2006"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12676"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-011-1023-7"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb06297.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.2996379"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.08.012"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2004.11.003"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509831103"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2007.00282.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123600"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00428367"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.65.1474"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02613-8"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/br.41.1.100-180.1977"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06519-11"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00414463"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050196r"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00029.x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(99)00065-1"},{"key":"e_1_2_6_48_1","volume-title":"Thermodynamics and Control of Biological Free\u2010Energy Transduction","author":"Westerhoff H. V.","year":"1987"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28849-0"},{"key":"e_1_2_6_50_1","volume-title":"Biology of Anaerobic Microorganisms","author":"Zehnder A. J. B.","year":"1989"},{"key":"e_1_2_6_51_1","unstructured":"Zhang T. Nevin K. Barlett M. Gannon S. Bain T.andLovley D.R.(2011) Anaerobic benzene degradation byGeobacterspecies with Fe(III) or an electrode as the electron acceptor. InThe 111th General Meeting of the American Society of Microbiology.New Orleans LA."},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.142"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1462-2920.14295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.14295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1462-2920.14295","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.14295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:19:42Z","timestamp":1761862782000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.14295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":51,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/1462-2920.14295"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.14295","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"2017-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}