{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:40:29Z","timestamp":1787013629088,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T00:00:00Z","timestamp":1537747200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31371891"],"award-info":[{"award-number":["31371891"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671975"],"award-info":[{"award-number":["31671975"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pyricularia oryzae<\/jats:italic>\n                    is a plant pathogen causing rice blast, a serious disease spreading in cultivated rice globally. Transcription factors play important regulatory roles in fungal development and pathogenicity. Here, we characterized the biological functions of Crf1, a basic helix\u2013loop\u2013helix (bHLH) transcription factor, in the development and pathogenicity of\n                    <jats:italic>P. oryzae<\/jats:italic>\n                    with functional genetics, molecular and biochemical approaches. We found that\n                    <jats:italic>CRF1<\/jats:italic>\n                    is necessary for virulence and plays an indispensable role in the regulation of carbohydrate and lipid metabolism in\n                    <jats:italic>P. oryzae<\/jats:italic>\n                    . Deletion of\n                    <jats:italic>CRF1<\/jats:italic>\n                    led to defects in utilization of lipids, ethanol, glycerol and L\u2010arabinose, and down\u2010regulation of many important genes in lipolysis,\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010oxidation, gluconeogenesis, as well as glycerol and arabinose metabolism.\n                    <jats:italic>CRF1<\/jats:italic>\n                    is also essential for peroxisome and vacuole function, and conidial cell death during appressorium formation. The appressorium turgor, penetration ability and virulence in \u0394\n                    <jats:italic>crf1<\/jats:italic>\n                    were restored by supplementation of exogenous glucose. The virulence of Crf1 mutant was also recovered by adding exogenous D\u2010xylose, but not by addition of ethanol, pyruvate, leucine or L\u2010arabinose. These data showed that Crf1 plays an important role in the complex regulatory network of carbohydrate and lipid metabolism that governs fungal development and pathogenicity.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14387","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T13:06:27Z","timestamp":1534770387000},"page":"3427-3441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The basic helix\u2013loop\u2013helix transcription factor Crf1 is required for development and pathogenicity of the rice blast fungus by regulating carbohydrate and lipid metabolism"],"prefix":"10.1111","volume":"20","author":[{"given":"Huijuan","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory for Rice Biology College of Life Sciences, Zhejiang University  Hangzhou 310058 Zhejiang Province China"},{"name":"Institute of Plant Protection, Jiangsu Academy of 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