{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T01:06:22Z","timestamp":1768525582296,"version":"3.49.0"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000936","name":"Gordon and Betty Moore Foundation","doi-asserted-by":"publisher","award":["3789"],"award-info":[{"award-number":["3789"]}],"id":[{"id":"10.13039\/100000936","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The model haloarchaeon,\n                    <jats:italic>Haloferax volcanii<\/jats:italic>\n                    possess an extremely high, and highly specific, basal caspase activity in exponentially growing cells that closely resembles caspase\u20104. This activity is specifically inhibited by the pan\u2010caspase inhibitor, z\u2010VAD\u2010FMK, and has no cross\u2010reactivity with other known protease families. Although it is one of the dominant cellular proteolytic activities in exponentially growing\n                    <jats:italic>H. volcanii<\/jats:italic>\n                    cells, the interactive cellular roles remain unknown and the protein(s) responsible for this activity remain elusive. Here, biochemical purification and\n                    <jats:italic>in situ<\/jats:italic>\n                    trapping with caspase targeted covalent inhibitors combined with genome\u2010enabled proteomics, structural analysis, targeted gene knockouts and treatment with canavanine demonstrated a catalytic linkage between caspase activity and thermosomes, proteasomes and cdc48b, a cell division protein and proteasomal degradation facilitating ATPase, as part of an \u2018interactase\u2019 of stress\u2010related protein complexes with an established link to the unfolded protein response (UPR). Our findings provide novel cellular and biochemical context for the observed caspase activity in\n                    <jats:italic>Archaea<\/jats:italic>\n                    and add new insight to understanding the role of this activity, implicating their possible role in the establishment of protein stress and ER associated degradation pathways in\n                    <jats:italic>Eukarya<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14456","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T00:09:23Z","timestamp":1540771763000},"page":"286-298","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Catalytic linkage between caspase activity and proteostasis in\n                    <i>Archaea<\/i>"],"prefix":"10.1111","volume":"21","author":[{"given":"Mansha","family":"Seth\u2010Pasricha","sequence":"first","affiliation":[{"name":"Department of Marine and Coastal Sciences Rutgers University  New Brunswick NJ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Senn","sequence":"additional","affiliation":[{"name":"Abteilung f\u00fcr Chemie und Bioanalytik Universit\u00e4t Salzburg  Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura E.","family":"Sanman","sequence":"additional","affiliation":[{"name":"Department of Pathology Stanford University School of Medicine  Stanford CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Bogyo","sequence":"additional","affiliation":[{"name":"Department of Pathology Stanford University School of Medicine  Stanford CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikas","family":"Nanda","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Robert Wood Johnson Medical School, Center for Advanced Biotechnology and Medicine, Rutgers University  Piscataway NJ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly A.","family":"Bidle","sequence":"additional","affiliation":[{"name":"Department of Biology, Behavioral Neuroscience, and Health Sciences Rider University  Lawrenceville NJ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4170-412X","authenticated-orcid":false,"given":"Kay D.","family":"Bidle","sequence":"additional","affiliation":[{"name":"Department of Marine and Coastal Sciences Rutgers University  New Brunswick NJ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.3.1791"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.2.943-953.2004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01341-3"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049888"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1224352"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom4030862"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7310112"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02157.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.11.3052"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/w05-147"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099104"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.1"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513331200"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80423-0"},{"key":"e_1_2_5_16_1","first-page":"197","volume-title":"Archaea: A Laboratory Manual","author":"Cline S. 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