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The opportunistic pathogen employs the Lqs (\n                    <jats:italic>Legionella<\/jats:italic>\n                    quorum sensing) system and the signalling molecule LAI\u20101 (\n                    <jats:italic>Legionella<\/jats:italic>\n                    autoinducer\u20101) to regulate virulence, motility, natural competence and expression of a 133 kb genomic \u201cfitness island\u201d, including a putative novel regulator. Here, we show that the regulator termed LvbR is an LqsS\u2010regulated transcription factor that binds to the promoter of\n                    <jats:italic>lpg<\/jats:italic>\n                    1056\/\n                    <jats:italic>hnox1<\/jats:italic>\n                    (encoding an inhibitor of the diguanylate cyclase Lpg1057\n                    <jats:italic>)<\/jats:italic>\n                    , and thus, regulates proteins involved in c\u2010di\u2010GMP metabolism. LvbR determines biofilm architecture, since\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    lacking\n                    <jats:italic>lvbR<\/jats:italic>\n                    accumulates less sessile biomass and forms homogeneous mat\u2010like structures, while the parental strain develops more compact bacterial aggregates. Comparative transcriptomics of sessile and planktonic \u0394\n                    <jats:italic>lvbR<\/jats:italic>\n                    or \u0394\n                    <jats:italic>lqsR<\/jats:italic>\n                    mutant strains revealed concerted (virulence, fitness island, metabolism) and reciprocally (motility) regulated genes in biofilm and broth respectively. Moreover, \u0394\n                    <jats:italic>lvbR<\/jats:italic>\n                    is hyper\u2010competent for DNA uptake, defective for phagocyte infection, outcompeted by the parental strain in amoebae co\u2010infections and impaired for cell migration inhibition. Taken together, our results indicate that\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    LvbR is a novel pleiotropic transcription factor, which links the Lqs and c\u2010di\u2010GMP regulatory networks to control biofilm architecture and pathogen\u2013host cell interactions.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14523","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T03:29:55Z","timestamp":1547004595000},"page":"1035-1053","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The pleiotropic\n                    <i>Legionella<\/i>\n                    transcription factor LvbR links the Lqs and c\u2010di\u2010GMP regulatory networks to control biofilm architecture and virulence"],"prefix":"10.1111","volume":"21","author":[{"given":"Ramon","family":"Hochstrasser","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, Faculty of Medicine, University of Z\u00fcrich  Gloriastrasse 30, 8006 Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Kessler","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute, Faculty of Medicine, Ludwig\u2010Maximilians University  Pettenkoferstrasse 9a, 80336 Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Sahr","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires  28 Rue du Dr Roux, 75724 Paris France"},{"name":"CNRS UMR 3525  28 Rue du Dr Roux, 75724 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Simon","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Faculty of Medicine, University of Z\u00fcrich  Gloriastrasse 30, 8006 Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Schell","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute, Faculty of Medicine, Ludwig\u2010Maximilians University  Pettenkoferstrasse 9a, 80336 Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gomez\u2010Valero","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires  28 Rue du Dr Roux, 75724 Paris France"},{"name":"CNRS UMR 3525  28 Rue du Dr Roux, 75724 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Buchrieser","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires  28 Rue du Dr Roux, 75724 Paris France"},{"name":"CNRS UMR 3525  28 Rue du Dr Roux, 75724 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5462-9301","authenticated-orcid":false,"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Faculty of Medicine, University of Z\u00fcrich  Gloriastrasse 30, 8006 Z\u00fcrich 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