{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T18:50:57Z","timestamp":1772823057721,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T00:00:00Z","timestamp":1558569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008321","name":"Pennsylvania State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004341","name":"Dow Chemical Company","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"Summary<\/jats:title>\n \n Microbiologically influenced corrosion causes $100 billion in damage per year, and biofilms formed by sulfate\u2010reducing bacteria (SRB) are the major culprit. However, little is known about the regulation of SRB biofilm formation. Using\n Desulfovibrio vulgaris<\/jats:italic>\n as a model SRB organism, we compared the transcriptomes of biofilm and planktonic cells and identified that the gene for \u03c3\n 54<\/jats:sub>\n \u2010dependent regulator DVU2956 is repressed in biofilms. Utilizing a novel promoter that is primarily transcribed in biofilms (P\n dvu0304<\/jats:sub>\n ), we found production of DVU2956 inhibits biofilm formation by 70%. Corroborating this result, deleting\n dvu2956<\/jats:italic>\n increased biofilm formation, and this biofilm phenotype could be complemented. By producing proteins in biofilms from genes controlled by DVU2956 (\n dvu2960<\/jats:italic>\n and\n dvu2962<\/jats:italic>\n ), biofilm formation was inhibited almost completely. A second round of RNA\u2010seq for the production of DVU2956 revealed DVU2956 influences electron transport via an Hmc complex (high\u2010molecular\u2010weight cytochrome\n c<\/jats:italic>\n encoded by\n dvu0531<\/jats:italic>\n \u2013\n dvu0536<\/jats:italic>\n ) and the Fe\u2010only hydrogenase (encoded by\n dvu1769<\/jats:italic>\n ,\n hydA<\/jats:italic>\n and\n dvu1770<\/jats:italic>\n ,\n hydB<\/jats:italic>\n ) to control H\n 2<\/jats:sub>\n S production. Corroborating these results, producing DVU2956 in biofilms decreased H\n 2<\/jats:sub>\n S production by half, deleting\n dvu2956<\/jats:italic>\n increased H\n 2<\/jats:sub>\n S production by 131 \u00b1 5%, and producing DVU2956 in the\n dvu2956<\/jats:italic>\n strain reduced H\n 2<\/jats:sub>\n S production. Therefore, DVU2956 maintains SRB in the planktonic state and reduces H\n 2<\/jats:sub>\n S formation.\n <\/jats:p>","DOI":"10.1111\/1462-2920.14679","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T06:30:20Z","timestamp":1557815420000},"page":"3564-3576","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["\u03c3\n 54<\/sub>\n \u2010Dependent regulator DVU2956 switches\n Desulfovibrio vulgaris<\/i>\n from biofilm formation to planktonic growth and regulates hydrogen sulfide production"],"prefix":"10.1111","volume":"21","author":[{"given":"Lei","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Pennsylvania State University University Park, State College PA 16802 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Pennsylvania State University University Park, State College PA 16802 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thammajun L.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Pennsylvania State University University Park, State College PA 16802 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryota","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Pennsylvania State University University Park, State College PA 16802 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6258-529X","authenticated-orcid":false,"given":"Thomas K.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Pennsylvania State University University Park, State College PA 16802 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