{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:35:24Z","timestamp":1790627724544,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T00:00:00Z","timestamp":1562198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["XDJK2016D057"],"award-info":[{"award-number":["XDJK2016D057"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872026"],"award-info":[{"award-number":["31872026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The ability to withstand heat and cell wall stress is critical for successful infection of target hosts by many pathogenic fungi. We report on the characterization of\n                    <jats:italic>BbThm1<\/jats:italic>\n                    (transcription factor for heat and membrane integrity), a Zn(II)\n                    <jats:sub>2<\/jats:sub>\n                    Cys\n                    <jats:sub>6<\/jats:sub>\n                    (Gal4\u2010like) family member, which regulates resistance to heat (32\u00b0C) and specific detergents in the insect pathogenic fungus,\n                    <jats:italic>Beauveria bassiana. BbThm1<\/jats:italic>\n                    gene knock\u2010out mutants showed severely impaired growth\/resistance at 32\u00b0C and to SDS, but mild to moderate phenotypic responses to other stresses including oxidative, osmotic and cell\u2010wall targeting compounds, or to various fungicides. Shifts in cell wall properties, adhesion and decreased viability were noted for the \u0394\n                    <jats:italic>BbThm1<\/jats:italic>\n                    mutant. Susceptibility to SDS but not heat could be rescued by exogenous ergosterol. Insect bioassays revealed decreased virulence of the \u0394\n                    <jats:italic>BbThm1<\/jats:italic>\n                    mutant against\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    both topically and via intrahemocoel injection assays that correlated with impaired\n                    <jats:italic>in insecta<\/jats:italic>\n                    hyphal body formation and altered host immune prophenol oxidase (PO) activation. Transcriptomic analyses of the wild\u2010type and \u0394\n                    <jats:italic>BbThm1<\/jats:italic>\n                    mutants revealed up\u2010regulation of hydrolases but down\u2010regulation of a wide range of basic metabolic processes. These data reveal fine tune aspects of the transcription factor network that regulates specific stress responses to contribute to the overall pathogenic process.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14718","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T11:43:44Z","timestamp":1560858224000},"page":"3153-3171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The Thm1 Zn(II)\n                    <sub>2<\/sub>\n                    Cys\n                    <sub>6<\/sub>\n                    transcription factor contributes to heat, membrane integrity and virulence in the insect pathogenic fungus\n                    <i>Beauveria bassiana<\/i>"],"prefix":"10.1111","volume":"21","author":[{"given":"Shuaishuai","family":"Huang","sequence":"first","affiliation":[{"name":"Biotechnology Research Center, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University Southwest University  Chongqing 400715 P. R. China"},{"name":"Department of Microbiology and Cell Science University of Florida  Gainesville Florida 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2855-5158","authenticated-orcid":false,"given":"Nemat O.","family":"Keyhani","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science University of Florida  Gainesville Florida 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University Southwest University  Chongqing 400715 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8101-4313","authenticated-orcid":false,"given":"Yongjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University Southwest University  Chongqing 400715 P. R. 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