{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:41:22Z","timestamp":1790772082424,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Bradyrhizobia are abundant soil bacteria, which can form nitrogen\u2010fixing symbioses with leguminous plants, including important crops such as soybean, cowpea and peanut. Many bradyrhizobia can denitrify, but studies have hitherto focused on a few model organisms. We screened 39 diverse\n                    <jats:italic>Bradyrhizobium<\/jats:italic>\n                    strains, isolated from legume nodules. Half of them were unable to reduce N\n                    <jats:sub>2<\/jats:sub>\n                    O, making them sources of this greenhouse gas. Most others could denitrify NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    to N\n                    <jats:sub>2<\/jats:sub>\n                    . Time\u2010resolved gas kinetics and transcription analyses during transition to anaerobic respiration revealed a common regulation of\n                    <jats:italic>nirK<\/jats:italic>\n                    ,\n                    <jats:italic>norCB<\/jats:italic>\n                    and\n                    <jats:italic>nosZ<\/jats:italic>\n                    (encoding NO\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    , NO and N\n                    <jats:sub>2<\/jats:sub>\n                    O reductases), and differing regulation of\n                    <jats:italic>napAB<\/jats:italic>\n                    (encoding periplasmic NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    reductase). A prominent feature in all N\n                    <jats:sub>2<\/jats:sub>\n                    \u2010producing strains was a virtually complete hampering of NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    reduction in the presence of N\n                    <jats:sub>2<\/jats:sub>\n                    O. In\u2010depth analyses suggest that this was due to a competition between electron transport pathways, strongly favouring N\n                    <jats:sub>2<\/jats:sub>\n                    O over NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    reduction. In a natural context, bacteria with this feature would preferentially reduce available N\n                    <jats:sub>2<\/jats:sub>\n                    O, produced by themselves or other soil bacteria, making them powerful sinks for this greenhouse gas. One way to augment such populations in agricultural soils is to develop inoculants for legume crops with dual capabilities of efficient N\n                    <jats:sub>2<\/jats:sub>\n                    \u2010fixation and efficient N\n                    <jats:sub>2<\/jats:sub>\n                    O reduction.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14731","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T13:44:15Z","timestamp":1562247855000},"page":"17-31","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["A common mechanism for efficient N\n                    <sub>2<\/sub>\n                    O reduction in diverse isolates of nodule\u2010forming bradyrhizobia"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0443-7667","authenticated-orcid":false,"given":"Daniel","family":"Mania","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Science  \u00e5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kedir","family":"Woliy","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Science  \u00e5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulu","family":"Degefu","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Science  \u00e5s Norway"},{"name":"International Crops Research Institute for the Semi\u2010Arid Tropics  Addis Ababa Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00e5sa","family":"Frosteg\u00e5rd","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Science  \u00e5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32869"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0330141"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00055-16"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01711.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2017.02.002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20150880"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3098"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac50052a028"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-128-8-1811"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2017.04.001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.049130-0"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26620-0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2010.06.004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(86)90064-2"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-008-9668-3"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06262-11"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nclimate1734"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(69)90461-5"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.03.014"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.12.012"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"8702","DOI":"10.1016\/S0021-9258(18)47471-6","article-title":"Identification 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