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Recently, small regulatory RNAs (sRNAs) have become increasingly recognized as important regulators of virulence gene expression in response to environmental signals. In this study, we determined that two\u2010component system EnvZ\/OmpR was required for intestinal colonization in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O1 EI Tor strain E12382. Analysis of the characteristics of OmpR revealed a potential binding site in the intergenic region between\n                    <jats:italic>vc1470<\/jats:italic>\n                    and\n                    <jats:italic>vc1471<\/jats:italic>\n                    , and qRT\u2010PCR showed that expression of the intergenic region increased 5.3\u2010fold in the small intestine compared to LB medium. Race and northern blot assays were performed and demonstrated a new sRNA,\n                    <jats:italic>coaR<\/jats:italic>\n                    (cholerae osmolarity and acidity related regulatory RNA). A \u0394\n                    <jats:italic>coaR<\/jats:italic>\n                    mutant showed a deficient colonization ability in small intestine with CI of 0.15. We identified a target of\n                    <jats:italic>coaR<\/jats:italic>\n                    ,\n                    <jats:italic>tcpI<\/jats:italic>\n                    , a negative regulator of the major pilin subunit of TcpA. The \u0394\n                    <jats:italic>tcpI<\/jats:italic>\n                    mutant has an increased colonization with CI of 3.16. The expression of\n                    <jats:italic>coaR<\/jats:italic>\n                    increased 2.8\u2010fold and 3.3\u2010fold under relative acidic and hypertonic condition. In summary,\n                    <jats:italic>coaR<\/jats:italic>\n                    was induced under the condition of high osmolarity and acid stress via EnvZ\/OmpR and explained that\n                    <jats:italic>tcpI<\/jats:italic>\n                    relieves pH\u2010mediated repression of toxin co\u2010regulated pilus synthesis.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14906","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T07:10:23Z","timestamp":1577085023000},"page":"4231-4243","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Small RNA\n                    <scp>\n                      <i>coaR<\/i>\n                    <\/scp>\n                    contributes to intestinal colonization in\n                    <scp>\n                      <i>Vibrio cholerae<\/i>\n                    <\/scp>\n                    via the two\u2010component system 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