{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:54:30Z","timestamp":1790700870747,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T00:00:00Z","timestamp":1580947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000146","name":"Alberta Innovates - Technology Futures","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007631","name":"Canadian Institute for Advanced Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007631","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Populations of the bacterium\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    consist of dozens of distinct lineages, with primarily (but not exclusively) members of the pandemic generating lineage capable of causing the diarrhoeal disease cholera. Assessing the composition and temporal dynamics of such populations requires extensive isolation efforts and thus only rarely covers large geographic areas or timeframes exhaustively. We developed a culture\u2010independent amplicon sequencing strategy based on the protein\u2010coding gene\n                    <jats:italic>viuB<\/jats:italic>\n                    (vibriobactin utilization) to study the structure of a\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    population over the course of a summer. We show that the 26 co\u2010occurring\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    lineages continuously compete for limited space on nutrient\u2010rich particles where only a few of them can grow to large numbers. Differential abundance of lineages between locations and size\u2010fractions associated with a particle\u2010attached or free\u2010swimming lifestyle could reflect adaptation to various environmental niches. In particular, a major\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    lineage occasionally grows to large numbers on particles but remain undetectable using isolation\u2010based methods, indicating selective culturability for some members of the species. We thus demonstrate that isolation\u2010based studies may not accurately reflect the structure and complex dynamics of\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    populations and provide a scalable high\u2010throughput method for both epidemiological and ecological approaches to studying this species.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14921","type":"journal-article","created":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T02:11:56Z","timestamp":1579745516000},"page":"4244-4256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Culture\u2010independent tracking of\n                    <i>Vibrio cholerae<\/i>\n                    lineages reveals complex spatiotemporal dynamics in a natural 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