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While high throughput metagenomic sequencing reveals genotypes of microbial communities, the functionality, phenotype and heterogeneity of human gut microbiota are still elusive. In this study, we applied Raman microscopy and deuterium isotope probing (Raman\u2013DIP) to detect metabolic active ARB (MA\u2010ARB)\n                    <jats:italic>in situ<\/jats:italic>\n                    at the single\u2010cell level in human gut microbiota from two healthy adults. We analysed the relative abundances of MA\u2010ARB under different concentrations of amoxicillin, cephalexin, tetracycline, florfenicol and vancomycin. To establish the link between phenotypes and genotypes of the MA\u2010ARB, Raman\u2010activated cell sorting (RACS) was used to sort MA\u2010ARB from human gut microbiota, and mini\u2010metagenomic DNA of the sorted bacteria was amplified, sequenced and analysed. The sorted MA\u2010ARB and their associated ARGs were identified. Our results suggest a strong relation between ARB in human gut microbiota and personal medical history. This study demonstrates that the toolkit of Raman\u2013DIP, RACS and DNA sequencing can be useful to unravel both phenotypes and genotypes of ARB in human gut microbiota at the single\u2010cell level.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.14962","type":"journal-article","created":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T19:50:10Z","timestamp":1583092210000},"page":"2613-2624","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Raman\u2010activated sorting of antibiotic\u2010resistant bacteria in human gut microbiota"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5701-9229","authenticated-orcid":false,"given":"Yi","family":"Wang","sequence":"first","affiliation":[{"name":"School of Environment Harbin Institute of Technology  Harbin 150090 China"},{"name":"Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of 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