{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:36:43Z","timestamp":1790793403578,"version":"4.1.0"},"reference-count":85,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T00:00:00Z","timestamp":1586304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["WA 2713\/4\u20102"],"award-info":[{"award-number":["WA 2713\/4\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The genome of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    , the etiological agent of cholera, is an exception to the single chromosome rule found in the vast majority of bacteria and has its genome partitioned between two unequally sized chromosomes. This unusual two\u2010chromosome arrangement in\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    has sparked considerable research interest since its discovery. It was demonstrated that the two chromosomes could be fused by deliberate genome engineering or forced to fuse spontaneously by blocking the replication of Chr2, the secondary chromosome. Recently, natural isolates of\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    with chromosomal fusion have been found. Here, we summarize the pertinent findings on this exception to the exception rule and discuss the potential utility of single\u2010chromosome\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    to address fundamental questions on chromosome biology in general and DNA replication in particular.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15002","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T03:10:23Z","timestamp":1585710623000},"page":"4123-4132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Exception to the exception rule: synthetic and naturally occurring single chromosome\n                    <i>Vibrio cholerae<\/i>"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6762-7874","authenticated-orcid":false,"given":"Shanmuga","family":"Sozhamannan","sequence":"first","affiliation":[{"name":"Defense Biological Product Assurance Office, CBRND\u2010Enabling Biotechnologies, 110 Thomas Johnson Drive  Frederick MD 21702 USA"},{"name":"Logistics Management Institute  Tysons VA 22102 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1505-9294","authenticated-orcid":false,"given":"Torsten","family":"Waldminghaus","sequence":"additional","affiliation":[{"name":"Department of Biology Technische Universit\u00e4t Darmstadt  Darmstadt Germany"},{"name":"Centre for Synthetic Biology Technische Universit\u00e4t Darmstadt  Darmstadt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.24.7869-7874.1993"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0689-y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004184"},{"key":"e_1_2_9_5_1","volume":"10","author":"Bhabatosh D.","year":"2019","journal-title":"Frontiersin 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