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Using whole\u2010genome single\u2010molecule real\u2010time (SMRT) sequencing, we characterized genomic variability of multidrug\u2010resistant\n                    <jats:italic>Rhodococcus equi<\/jats:italic>\n                    isolated from soil samples from 100 farms endemic for\n                    <jats:italic>R<\/jats:italic>\n                    .\n                    <jats:italic>equi<\/jats:italic>\n                    infections in Kentucky. We discovered the novel\n                    <jats:italic>erm<\/jats:italic>\n                    (51)\u2010encoding resistance to MLS\n                    <jats:sub>B<\/jats:sub>\n                    in\n                    <jats:italic>R<\/jats:italic>\n                    .\n                    <jats:italic>equi<\/jats:italic>\n                    isolates from soil of horse\u2010breeding farms.\n                    <jats:italic>Erm<\/jats:italic>\n                    (51) is inserted in a transposon (Tn\n                    <jats:italic>Erm<\/jats:italic>\n                    51) that is associated with a putative conjugative plasmid (pRErm51), a mobilizable plasmid (pMobErm51), or both enabling horizontal gene transfer to susceptible organisms and conferring high levels of resistance against MLS\n                    <jats:sub>B<\/jats:sub>\n                    in vitro. This new resistant genotype also carries a previously unidentified\n                    <jats:italic>rpoB<\/jats:italic>\n                    mutation conferring resistance to rifampicin. Isolates carrying both\n                    <jats:italic>vapA<\/jats:italic>\n                    and\n                    <jats:italic>erm<\/jats:italic>\n                    (51) were rarely found, indicating either a recent acquisition of\n                    <jats:italic>erm<\/jats:italic>\n                    (51) and\/or impaired survival when isolates carry both genes. Isolates carrying\n                    <jats:italic>erm<\/jats:italic>\n                    (51) are closely related genetically and were likely selected by antimicrobial exposure in the environment.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15020","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T01:04:02Z","timestamp":1586912642000},"page":"2858-2869","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The novel and transferable\n                    <scp>\n                      <i>erm<\/i>\n                    <\/scp>\n                    (51) gene confers macrolides, lincosamides and streptogramins B (\n                    <scp>\n                      MLS\n                      <sub>B<\/sub>\n                    <\/scp>\n                    ) resistance to clonal\n                    <scp>\n                      <i>Rhodococcus equi<\/i>\n                    <\/scp>\n                    in the environment"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5138-0336","authenticated-orcid":false,"given":"Laura","family":"Huber","sequence":"first","affiliation":[{"name":"Department of Large Animal Medicine, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steeve","family":"Gigu\u00e8re","sequence":"additional","affiliation":[{"name":"Department of Large Animal Medicine, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan M.","family":"Slovis","sequence":"additional","affiliation":[{"name":"Hagyard Equine Medical Institute  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonsiray","family":"\u00c1lvarez\u2010Narv\u00e1ez","sequence":"additional","affiliation":[{"name":"Department of Large Animal Medicine, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey A.","family":"Hart","sequence":"additional","affiliation":[{"name":"Department of Large Animal Medicine, College of Veterinary Medicine University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maggie","family":"Greiter","sequence":"additional","affiliation":[{"name":"Hagyard Equine Medical Institute  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7957-4642","authenticated-orcid":false,"given":"Ellen Ruth A.","family":"Morris","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0384-2903","authenticated-orcid":false,"given":"Noah D.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2005.pto960401.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02260-19"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-57479-9"},{"key":"e_1_2_7_5_1","first-page":"3184","article-title":"Novel transferable erm(46) determinant responsible for emerging macrolide resistance in Rhodococcus equi","volume":"70","author":"Anastasi E.","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw080"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux053"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3439"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02673-14"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1902.121210"},{"key":"e_1_2_7_11_1","unstructured":"M. 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