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In the case of the environmental opportunistic pathogen\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , it has been shown that overexpression of different efflux pumps is linked to the impairment of the quorum sensing (QS) response. Nevertheless, the causes of such impairment are different for each analysed efflux pump. Herein, we performed an in\u2010depth analysis of the QS\u2010mediated response of a\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    antibiotic resistant mutant that overexpresses MexAB\u2010OprM. Although previous work claimed that this efflux pump extrudes the QS signal 3\u2010oxo\u2010C12\u2010HSL, we show otherwise. Our results evidence that the observed attenuation in the QS response when overexpressing this pump is related to an impaired production of alkyl quinolone QS signals, likely prompted by the reduced availability of one of their precursors, the octanoate. Together with previous studies, this indicates that, although the consequences of overexpressing efflux pumps are similar (impaired QS response), the underlying mechanisms are different. This 'apparent redundancy' of MDR efflux systems can be understood as a\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    strategy to keep the robustness of the QS regulatory network and modulate its output in response to different signals.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15177","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T01:32:11Z","timestamp":1595813531000},"page":"5167-5188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["The impaired quorum sensing response of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    MexAB\u2010OprM efflux pump overexpressing mutants is not due to non\u2010physiological efflux of 3\u2010oxo\u2010C12\u2010HSL"],"prefix":"10.1111","volume":"22","author":[{"given":"Manuel","family":"Alcalde\u2010Rico","sequence":"first","affiliation":[{"name":"Centro Nacional de Biotecnolog\u00eda, CSIC  Madrid 28049 Spain"},{"name":"Grupo de Resistencia Antimicrobiana en Bacterias Pat\u00f3genas y Ambientales GRABPA, Instituto de Biolog\u00eda, Facultad de Ciencias, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valparaiso 2340025 Chile"},{"name":"Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB\u2010R)  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6841-7182","authenticated-orcid":false,"given":"Jorge","family":"Olivares\u2010Pacheco","sequence":"additional","affiliation":[{"name":"Grupo de Resistencia Antimicrobiana en Bacterias Pat\u00f3genas y Ambientales GRABPA, Instituto de Biolog\u00eda, Facultad de Ciencias, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valparaiso 2340025 Chile"},{"name":"Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB\u2010R)  Santiago 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