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Apart from phage \u03bb, little is known about other phages that target\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB. Here, we developed a simple method for isolating novel environmental phages in a predictable way, i.e. isolating phages that target a particular receptor(s) of a bacterium, in this case, the\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB protein. A plasmid (pMUT13) encoding the\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB porin was transferred into three different enterobacterial genera. By enrichment with these engineered bacteria, a number of phages (ZZ phages) that targeted\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB were easily isolated from the environment. Interestingly, although\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB\u2010dependent in their recombinant heterologous hosts, these newly isolated ZZ phages also targeted OmpC as an alternative receptor when infecting\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    . Moreover, the phage host range was readily extended within three different bacterial genera with heterologously expressed\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB. Unlike phage \u03bb, which is a member of the\n                    <jats:italic>Siphoviridae<\/jats:italic>\n                    family, these newly isolated\n                    <jats:italic>Ec<\/jats:italic>\n                    LamB\u2010dependent phages were more commonly members of the\n                    <jats:italic>Myoviridae<\/jats:italic>\n                    family, based on transmission electron microscopy and genomic sequences. Modifications of this convenient and efficient phage enrichment method could be useful for the discovery of novel phages.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15188","type":"journal-article","created":{"date-parts":[[2020,8,9]],"date-time":"2020-08-09T22:32:08Z","timestamp":1597012328000},"page":"5207-5221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Bacteriophage host range evolution through engineered enrichment bias, exploiting heterologous surface receptor expression"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4676-1468","authenticated-orcid":false,"given":"Ziyue","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5197-2198","authenticated-orcid":false,"given":"George P. C.","family":"Salmond","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(08)60785-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(03)00067-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9040070"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1011218107"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.38.1.372-375.1981"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw002"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.139.2.680-682.1979"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti553"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.47"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.53.2.667-671.1985"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.176.11.3204-3209.1994"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/v4113162"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.19.6080"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-031413-085500"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-7-290"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/382539"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00332415"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.169.5.2103-2106.1987"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1985.tb00664.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(86)90053-7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph12010035"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(81)80605-9"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00850"},{"key":"e_1_2_6_27_1","first-page":"205","article-title":"Phage 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