{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:09:46Z","timestamp":1790726986757,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,9,12]],"date-time":"2020-09-12T00:00:00Z","timestamp":1599868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Resistance Nodulation cell Division (RND) efflux pumps are known to contribute to the tolerance of\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    to aromatic hydrocarbons, but their role in antibiotic resistance has not been fully elucidated. In this study, two types of single\u2010step multidrug\u2010resistant (MDR) mutants were selected\n                    <jats:italic>in vitro<\/jats:italic>\n                    from reference strain KT2440. Mutants of the first type were more resistant to fluoroquinolones and \u03b2\u2010lactams except imipenem, and overproduced the efflux system TtgABC as a result of mutations occurring in regulator TtgR. In addition to TtgABC, mutants of the second type such as HPG\u20105 were found to upregulate a novel RND pump, dubbed ParXY\/TtgC, which accommodates cefepim, fluoroquinolones and aminoglycosides. As demonstrated by gene deletion experiments, TtgABC and ParXY\/TtgC are both under the positive control of a two\u2010component system, PpeRS. Whole\u2010genome sequence analyses revealed that mutant HPG\u20105 harbours a mutation inactivating the gene (\n                    <jats:italic>sucD<\/jats:italic>\n                    ) of succinyl\u2010CoA synthetase, an enzyme of the tricarboxylic cycle. Disruption of\n                    <jats:italic>sucD<\/jats:italic>\n                    in strain KT2440 reproduced the resistance phenotype of HPG\u20105, and activated the glyoxylate shunt. Finally, identification of two MDR clinical strains of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>putida<\/jats:italic>\n                    that jointly overexpress TtgABC and ParXY\/TtgC, of which one is a\n                    <jats:italic>sucD<\/jats:italic>\n                    mutant, highlights the role of these efflux systems as determinants of antibiotic resistance.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15200","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T02:28:38Z","timestamp":1598322518000},"page":"5222-5231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Coordinate overexpression of two\n                    <scp>RND<\/scp>\n                    efflux systems,\n                    <scp>ParXY<\/scp>\n                    and\n                    <scp>TtgABC<\/scp>\n                    , is responsible for multidrug resistance in\n                    <i>Pseudomonas putida<\/i>"],"prefix":"10.1111","volume":"22","author":[{"given":"H\u00e9l\u00e8ne","family":"Puja","sequence":"first","affiliation":[{"name":"UMR 6249 Chrono\u2010environnement UFR Sant\u00e9, Universit\u00e9 de Bourgogne\u2010Franche Comt\u00e9  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendoline","family":"Comment","sequence":"additional","affiliation":[{"name":"UMR 6249 Chrono\u2010environnement UFR Sant\u00e9, Universit\u00e9 de Bourgogne\u2010Franche Comt\u00e9  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Chassagne","sequence":"additional","affiliation":[{"name":"UMR 6249 Chrono\u2010environnement UFR Sant\u00e9, Universit\u00e9 de Bourgogne\u2010Franche Comt\u00e9  Besan\u00e7on 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