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Previous work has deciphered important regulatory circuits involved in natural competence induction as well as mechanistic details related to its DNA acquisition and uptake potential. However, since competence was first reported for\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    in 2005, many researchers have struggled with reproducibility in certain strains. In this study, we therefore compare prominent seventh pandemic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    isolates, namely strains A1552, N16961, C6706, C6709, E7946, P27459, and the close relative MO10, for their natural transformability and decipher underlying defects that mask the high degree of competence conservation. Through a combination of experimental approaches and comparative genomics based on new whole\u2010genome sequences and\n                    <jats:italic>de novo<\/jats:italic>\n                    assemblies, we identify several strain\u2010specific defects, mostly in genes that encode key players in quorum sensing. Moreover, we provide evidence that most of these deficiencies might have recently occurred through laboratory domestication events or through the acquisition of mobile genetic elements. Lastly, we highlight that differing experimental approaches between research groups might explain more of the variations than strain\u2010specific alterations.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15214","type":"journal-article","created":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T03:33:11Z","timestamp":1598671991000},"page":"4149-4166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Comparison of chitin\u2010induced natural transformation in pandemic\n                    <i>Vibrio cholerae<\/i>\n                    <scp>O1<\/scp>\n                    El Tor strains"],"prefix":"10.1111","volume":"22","author":[{"given":"Sandrine","family":"Stutzmann","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Microbiology, Global Health Institute School of Life Sciences, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL)  Lausanne CH\u20101015 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7024-1489","authenticated-orcid":false,"given":"Melanie","family":"Blokesch","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology, Global Health Institute School of Life Sciences, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL)  Lausanne CH\u20101015 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