{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T18:42:56Z","timestamp":1774204976855,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01ES023779"],"award-info":[{"award-number":["R01ES023779"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM55425"],"award-info":[{"award-number":["R01GM55425"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35 GM136211"],"award-info":[{"award-number":["R35 GM136211"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970108"],"award-info":[{"award-number":["31970108"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41967023"],"award-info":[{"award-number":["41967023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Organoarsenicals enter the environment from biogenic and anthropogenic sources. Trivalent inorganic arsenite (As(III)) is microbially methylated to more toxic methylarsenite (MAs(III)) and dimethylarsenite (DMAs(III)) that oxidize in air to MAs(V) and DMAs(V). Sources include the herbicide monosodium methylarsenate (MSMA or MAs(V)), which is microbially reduced to MAs(III), and the aromatic arsenical roxarsone (3\u2010nitro\u20104\u2010hydroxybenzenearsonic acid or Rox), an antimicrobial growth promoter for poultry and swine. Here we show that\n                    <jats:italic>Sphingobacterium wenxiniae<\/jats:italic>\n                    LQY\u201018\n                    <jats:sup>T<\/jats:sup>\n                    , isolated from activated sludge, is resistant to trivalent MAs(III) and Rox(III).\n                    <jats:italic>Sphingobacterium wenxiniae<\/jats:italic>\n                    detoxifies MAs(III) and Rox(III) by oxidation to MAs(V) and Rox(V).\n                    <jats:italic>Sphingobacterium wenxiniae<\/jats:italic>\n                    has a novel chromosomal gene, termed\n                    <jats:italic>arsU1<\/jats:italic>\n                    . Expressed in\n                    <jats:italic>Escherichia coli arsU1<\/jats:italic>\n                    confers resistance to MAs(III) and Rox(III) but not As(III) or pentavalent organoarsenicals. Purified ArsU1 catalyses oxidation of trivalent methylarsenite and roxarsone. ArsU1 has six conserved cysteine residues. The DNA sequence for the three C\u2010terminal cysteines was deleted, and the other three were mutated to serines. Only C45S and C122S lost activity, suggesting that Cys45 and Cys122 play a role in ArsU1 function. ArsU1 requires neither FMN nor FAD for activity. These results demonstrate that ArsU1 is a novel MAs(III) oxidase that contributes to\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>wenxiniae<\/jats:italic>\n                    tolerance to organoarsenicals.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15599","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:43:25Z","timestamp":1621212205000},"page":"762-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Organoarsenical tolerance in\n                    <i>Sphingobacterium wenxiniae<\/i>\n                    , a bacterium isolated from activated sludge"],"prefix":"10.1111","volume":"24","author":[{"given":"Jian","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cellular Biology and Pharmacology Herbert Wertheim College of Medicine, Florida International University  Miami FL 33199 USA"},{"name":"Institute of Environment Remediation and Human Health, and College of Ecology and Environment, Southwest Forestry University  Kunming 650224 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1965-7224","authenticated-orcid":false,"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Pharmacology Herbert Wertheim College of Medicine, Florida International University  Miami FL 33199 USA"},{"name":"Jiangsu Key Laboratory for Organic Waste Utilization Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University  Nanjing 210095 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5230-4271","authenticated-orcid":false,"given":"Barry 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