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Subinhibitory concentrations of antimicrobial peptides can modulate resistance, virulence and persistence effectors in Gram\u2010negative pathogens. In this study, we investigated the effect of subinhibitory concentrations of polymyxin B (PmB) on the secretome of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    , a natural inhabitant of aquatic environments and the pathogen responsible for the cholera disease. Our proteomic approach revealed that the abundance of many extracellular proteins is affected by PmB and some of them are detected only either in the presence or in the absence of PmB. The type VI secretion system (T6SS) secreted hemolysin\u2010coregulated protein (Hcp) displayed an increased abundance in the presence of PmB. Hcp is also more abundant in the bacterial cells in the presence of PmB and\n                    <jats:italic>hcp<\/jats:italic>\n                    expression is upregulated upon PmB supplementation. No effect of the T6SS on antimicrobial resistance was observed. Conversely, PmB increases the T6SS\u2010dependent cytotoxicity of\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    towards the amoeba\n                    <jats:italic>Dictyostelium discoideum<\/jats:italic>\n                    and its ability to compete with\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15762","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T07:59:38Z","timestamp":1631001578000},"page":"1133-1149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Secretome analysis reveals a role of subinhibitory concentrations of polymyxin B in the survival of\n                    <scp>\n                      <i>Vibrio cholerae<\/i>\n                    <\/scp>\n               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