{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:15:32Z","timestamp":1790831732963,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T00:00:00Z","timestamp":1632182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["322669"],"award-info":[{"award-number":["322669"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>The Dallol protovolcanic area on the Danakil Depression (Afar region, Ethiopia) exhibits unique hydrothermal manifestations in hypersaline context, yielding varied polyextreme physicochemical conditions. Previous studies identified a wide archaeal diversity in less extreme brines but failed to identify microorganisms thriving in either high\u2010chaotropicity, low\u2010water\u2010activity brines or hyperacidic\u2010hypersaline Na\u2010Fe\u2010rich brines. Recently, we accessed several small lakes under intense degassing activity adjacent to the Round Mountain, west to the Dallol dome [Western Canyon Lakes (WCL); WCL1\u20105]. They exhibited intermediate parameter combinations (pH\u00a0~\u20095, 34%\u201341% (weight\/volume) NaCl\u2010dominated salts with relatively high levels of chaotropic Mg\u2010Ca salts) that should allow to better constrain life limits. These lakes were overwhelmingly dominated by Archaea, encompassing up to 99% of prokaryotic 16S rRNA gene amplicon sequences in metabarcoding studies. The majority belonged to Halobacteriota and Nanohaloarchaeota, the latter representing up to half of prokaryotic sequences. Optical and epifluorescence microscopy showed active cells in natural samples and diverse morphotypes in enrichment cultures. Scanning electron microscopy coupled with energy\u2010dispersive X\u2010ray spectroscopy revealed tiny cells (200\u2013300\u2009nm diameter) epibiotically associated with somewhat larger cells (0.6\u20131\u00a0\u03bcm) but also the presence of silica\u2010dominated precipitates of similar size and shape, highlighting the difficulty of distinguishing microbes from mineral biomorphs in this kind of low\u2010biomass systems.<\/jats:p>","DOI":"10.1111\/1462-2920.15771","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T04:07:46Z","timestamp":1631592466000},"page":"7168-7182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Archaeal overdominance close to life\u2010limiting conditions in geothermally influenced hypersaline lakes at the Danakil Depression, Ethiopia"],"prefix":"10.1111","volume":"23","author":[{"given":"Jodie","family":"Belilla","sequence":"first","affiliation":[{"name":"Ecologie Syst\u00e9matique Evolution, CNRS Universit\u00e9 Paris\u2010Sud, Universit\u00e9 Paris\u2010Saclay, AgroParisTech  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Iniesto","sequence":"additional","affiliation":[{"name":"Ecologie Syst\u00e9matique Evolution, CNRS Universit\u00e9 Paris\u2010Sud, Universit\u00e9 Paris\u2010Saclay, AgroParisTech  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Moreira","sequence":"additional","affiliation":[{"name":"Ecologie Syst\u00e9matique Evolution, CNRS Universit\u00e9 Paris\u2010Sud, Universit\u00e9 Paris\u2010Saclay, AgroParisTech  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Benzerara","sequence":"additional","affiliation":[{"name":"Institut de Min\u00e9ralogie, de Physique des Mat\u00e9riaux et de Cosmochimie, CNRS Sorbonne Universit\u00e9, Mus\u00e9um National d'Histoire Naturelle  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"L\u00f3pez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Geol\u00f3gico y Minero de Espa\u00f1a, IGME_CSIC  Palma de Mallorca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana I.","family":"L\u00f3pez\u2010Archilla","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Reboul","sequence":"additional","affiliation":[{"name":"Ecologie Syst\u00e9matique Evolution, CNRS Universit\u00e9 Paris\u2010Sud, Universit\u00e9 Paris\u2010Saclay, AgroParisTech  Orsay 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