{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T05:36:02Z","timestamp":1783748162247,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["NAHEP\/CAAST\/2018\u201019"],"award-info":[{"award-number":["NAHEP\/CAAST\/2018\u201019"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>in vitro<\/jats:italic>\n                    antibacterial efficacy of an in\u2010house designed cell\u2010penetrating peptide (CPP) variant of Cecropin A (1\u20137)\u2010Melittin (CAMA) (CAMA\u2010CPP) against the characterized multi\u2010drug resistant (MDR) field strains of\n                    <jats:italic>Salmonella Enteritidis<\/jats:italic>\n                    and\n                    <jats:italic>Salmonella Typhimurium<\/jats:italic>\n                    were evaluated and compared with two identified CPPs namely, P7 and APP, keeping CAMA as control. Initially, the minimum inhibitory concentration (MIC) (\u03bcg\u2009ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) of in\u2010house designed CAMA\u2010CPP, APP and CAMA was determined to be 3.91, whereas that of P7 was 7.81; however, the minimum bactericidal concentration (MBC) of all the peptides were twice the MIC. CAMA\u2010CPP and CAMA were found to be stable under different conditions (high\u2010end temperatures, proteinase\u2010K, cationic salts, pH and serum) when compared to the other CPPs. Moreover, CAMA\u2010CPP exhibited negligible cytotoxicity in HEp\u20102 and RAW 264.7 cell lines as well as haemolysis in the sheep and human erythrocytes with no adverse effects against the commensal gut lactobacilli.\n                    <jats:italic>In vitro<\/jats:italic>\n                    time\u2010kill assay revealed that the MBC levels of CAMA\u2010CPP and APP could eliminate the intracellular MDR\u2010\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infections from mammalian cell lines; however, CAMA and P7 peptides were ineffective. CAMA\u2010CPP appears to be a promising antimicrobial candidate and opens up further avenues for its\n                    <jats:italic>in vivo<\/jats:italic>\n                    clinical translation.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15778","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T04:11:38Z","timestamp":1631765498000},"page":"2747-2758","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antibacterial efficacy of in\u2010house designed cell\u2010penetrating peptide against multi\u2010drug resistant strains of\n                    <scp>\n                      <i>Salmonella Enteritidis<\/i>\n                    <\/scp>\n                    and\n                    <scp>\n                      <i>Salmonella Typhimurium<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"24","author":[{"given":"Maria Anto Dani","family":"Nishanth","sequence":"first","affiliation":[{"name":"Division of Veterinary Public Health, ICAR\u2010Indian Veterinary Research Institute  Izatnagar Uttar Pradesh 243 122 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirsant","family":"Bhoomika","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR\u2010Indian Veterinary Research Institute  Izatnagar Uttar Pradesh 243 122 India"},{"name":"Department of Veterinary Public Health Bihar Veterinary College, Bihar Animal Sciences University  Patna Bihar 800 014 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diksha","family":"Gourkhede","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR\u2010Indian Veterinary Research Institute  Izatnagar Uttar Pradesh 243 122 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhargavi","family":"Dadimi","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR\u2010Indian Veterinary Research Institute  Izatnagar Uttar Pradesh 243 122 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4276-1129","authenticated-orcid":false,"given":"Jess","family":"Vergis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode Kerala Veterinary and Animal Sciences University  Wayanad Kerala 673 576 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satya Veer Singh","family":"Malik","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR\u2010Indian Veterinary Research Institute  Izatnagar Uttar Pradesh 243 122 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6543-9757","authenticated-orcid":false,"given":"Sukhadeo Baliram","family":"Barbuddhe","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Meat  Hyderabad Telangana 500 092 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-4708","authenticated-orcid":false,"given":"Deepak Bhiwa","family":"Rawool","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR\u2010Indian Veterinary Research Institute  Izatnagar Uttar Pradesh 243 122 India"},{"name":"ICAR\u2010National Research Centre on Meat  Hyderabad Telangana 500 092 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-019-01540-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/9789811461835120030003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25173858"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2019.106908"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.02.030"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2017.02.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41429-019-0148-1"},{"key":"e_1_2_7_9_1","unstructured":"Centers for Disease Control and Prevention (CDC)(2020) Salmonella. URLhttps:\/\/www.cdc.gov\/salmonella\/index.html"},{"key":"e_1_2_7_10_1","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. M07 Standard","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450024605445"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2015.11.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9020325"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200703183"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.36062\/ijah.59.1.2020.23-28"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-58259-3_2"},{"key":"e_1_2_7_17_1","unstructured":"European Food Safety Authority. (2019)Screening for salmonellosis. URLhttps:\/\/www.efsa.europa.eu\/en\/search\/site\/salmonellosis."},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2005.07.001"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.06.070"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ejihpe11010006"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104405"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69995-9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky386"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-010-0004-3"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22111929"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom8010004"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4352-1"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.05.026"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-012-1457-x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-021-10182-3"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07440-0"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00310"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2015.669-673"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.0c00393"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.6.949"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.11.014"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001067"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.3300"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2016-0088"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/fty072"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110649200"},{"key":"e_1_2_7_43_1","first-page":"343","volume-title":"Antimicrobial Resistance in Agriculture: Perspective, Policy and Mitigation","author":"Samanta I.","year":"2019"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.563"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.29303"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2018.01.066"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0481"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10071192"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071752"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.12.025"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CY00110G"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-020-01476-3"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02173"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftab010"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02723"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.575917"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00063-13"},{"key":"e_1_2_7_58_1","unstructured":"World Health Organization. (2020)Screening for salmonellosis most common foodborne illness. URLhttps:\/\/www.who.int\/topics\/foodborne_diseases\/en\/."},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.1120"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.15778","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1462-2920.15778","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.15778","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T06:47:50Z","timestamp":1761893270000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.15778"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,23]]},"references-count":58,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/1462-2920.15778"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.15778","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,23]]},"assertion":[{"value":"2021-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}