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An analysis of GNB with co\u2010resistance to carbapenems and polymyxins from a One Health perspective is presented. Data of species name, country, source of isolation, resistance genes (ARGs), plasmid type, clones and mobile genetic elements (MGEs) were deduced from 129 articles from January 2016 to March 2021. Available genomes and plasmids were obtained from PATRIC and NCBI. Resistomes and methylomes were analysed using BAcWGSTdb and REBASE whilst Kaptive was used to predict capsule typing. Plasmids and other MEGs were identified using MGE Finder and ResFinder. Phylogenetic analyses were done using RAxML and annotated with MEGA 7. A total of 877 isolates, 32 genomes and 44 plasmid sequences were analysed. Most of these isolates were reported in Asian countries and were isolated from clinical, animal and environmental sources. Colistin resistance was mostly mediated by\n                    <jats:italic>mgrB<\/jats:italic>\n                    inactivation (37%;\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u2009322) and\n                    <jats:italic>mcr\u20101<\/jats:italic>\n                    (36%;\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u2009312), while OXA\u201048\/181 was the most reported carbapenemase. IncX and IncI were the most common plasmids hosting carbapenemases and\n                    <jats:italic>mcr<\/jats:italic>\n                    genes. The isolates were co\u2010resistant to other antibiotics, with\n                    <jats:italic>floR<\/jats:italic>\n                    (chloramphenicol) and\n                    <jats:italic>fosA3<\/jats:italic>\n                    (fosfomycin) being common;\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    ST156 and\n                    <jats:italic>K<\/jats:italic>\n                    .\n                    <jats:italic>pneumoniae<\/jats:italic>\n                    ST258 strains were common globally. Virulence genes and capsular KL\u2010types were also detected. Type I, II, III and IV restriction modification systems were detected, comprising various MTases and restriction enzymes. The escalation of highly resistant isolates drains the economy due to untreatable bacterial infections, which leads to increasing global mortality rates and healthcare costs.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15930","type":"journal-article","created":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T05:55:24Z","timestamp":1644213324000},"page":"1518-1542","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Phylogenomics, epigenomics, virulome and mobilome of Gram\u2010negative bacteria co\u2010resistant to carbapenems and polymyxins: a\n                    <scp>One Health<\/scp>\n                    systematic review and meta\u2010analyses"],"prefix":"10.1111","volume":"24","author":[{"given":"Winnie Thabisa","family":"Ramaloko","sequence":"first","affiliation":[{"name":"Department of Dermatology, School of Medicine, Faculty of Health Sciences University of Pretoria  Pretoria South 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