{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:34:05Z","timestamp":1792852445524,"version":"4.5.0"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L026074\/1"],"award-info":[{"award-number":["BB\/L026074\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T009152\/1"],"award-info":[{"award-number":["BB\/T009152\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["726116"],"award-info":[{"award-number":["726116"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Bacteria possess various regulatory mechanisms to detect and coordinate a response to elemental nutrient limitation. In pseudomonads, the two\u2010component system regulators CbrAB, NtrBC and PhoBR, are responsible for regulating cellular response to carbon (C), nitrogen (N) and phosphorus (P) respectively. Phosphonates are reduced organophosphorus compounds produced by a broad range of biota and typified by a direct C\u2010P bond. Numerous pseudomonads can use the environmentally abundant phosphonate species 2\u2010aminoethylphosphonate (2AEP) as a source of C, N, or P, but only PhoBR has been shown to play a role in 2AEP utilization. On the other hand, utilization of 2AEP as a C and N source is considered substrate inducible. Here, using the plant\u2010growth\u2010promoting rhizobacterium\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    BIRD\u20101 we present evidence that 2AEP utilization is under dual regulation and only occurs upon depletion of C, N, or P, controlled by CbrAB, NtrBC, or PhoBR respectively. However, the presence of 2AEP was necessary for full gene expression, i.e. expression was substrate inducible. Mutation of a LysR\u2010type regulator, termed AepR, upstream of the 2AEP transaminase\u2010phosphonatase system (PhnWX), confirmed this dual regulatory mechanism. To our knowledge, this is the first study identifying coordination between global stress response and substrate\u2010specific regulators in phosphonate metabolism.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.15959","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T04:44:49Z","timestamp":1646109889000},"page":"1902-1917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["2\u2010Aminoethylphosphonate utilization in\n                    <i>Pseudomonas putida<\/i>\n                    \u2009\n                    <scp>BIRD<\/scp>\n                    \u20101 is controlled by multiple master regulators"],"prefix":"10.1111","volume":"24","author":[{"given":"Andrew R. J.","family":"Murphy","sequence":"first","affiliation":[{"name":"School of Life Sciences University of Warwick, Gibbet Hill Road  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3093-4245","authenticated-orcid":false,"given":"David J.","family":"Scanlan","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick, Gibbet Hill Road  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0367-4276","authenticated-orcid":false,"given":"Yin","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick, Gibbet Hill Road  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary D.","family":"Bending","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick, Gibbet Hill Road  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Hammond","sequence":"additional","affiliation":[{"name":"School of Agriculture, Policy, and Development University of Reading, Earley Gate, Whiteknights  Reading UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M. H.","family":"Wellington","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick, Gibbet Hill Road  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7190-315X","authenticated-orcid":false,"given":"Ian D. E. A.","family":"Lidbury","sequence":"additional","affiliation":[{"name":"Plants, Photosynthesis and Soil Research Cluster, School of Biosciences University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9071419"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00773"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01015-12"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00131a026"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12531"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.237735"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/es061843e"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.01.010"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-017-0031-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/30881"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0026261711030076"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2017.09.025"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2018.10.011"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12270"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-102215-095331"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00744-09"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01230-07"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.22.6411-6421.1995"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-013-1375-2"},{"key":"e_1_2_6_21_1","volume-title":"Phosphonates: Their Natural Occurrence and Physiological Role","author":"Kafarski P.","year":"2019"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.15.4134-4140.2002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2001.46.2.0309"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.11.3268-3275.2001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01477.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8050725"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.06.026"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.61.080706.093409"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmicro\/54.suppl_1.i43"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000781"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00432-07"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02327-6"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13390"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1317834111"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02062.x"},{"key":"e_1_2_6_36_1","volume-title":"Experiments in Molecular Genetics","author":"Miller J.H.","year":"1972"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.3.1910-1924.2006"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45554-9"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07912.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12499"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1002804409503"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24646-z"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02435.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2013.06.018"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01489"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-015-2495-4"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00218.x"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00402"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo4010001"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910308106"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0038-0717(82)90022-0"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(98)80043-X"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.79"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2004.01.011"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.136"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.078873-0"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00019"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02264.x"},{"key":"e_1_2_6_60_1","first-page":"1357","volume-title":"Escherichia coli and Salmonella: Cellular and Molecular Biology","author":"Wanner B.L.","year":"1996"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5876-5882.2004"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111501"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00799-9"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00911-10"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10498-009-9087-y"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315107110"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.1c00092"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.081984"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-021-03144-w"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.08.003"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.15959","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1462-2920.15959","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.15959","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T03:54:20Z","timestamp":1761882860000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.15959"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,8]]},"references-count":69,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1111\/1462-2920.15959"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.15959","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,8]]},"assertion":[{"value":"2021-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}