{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T01:23:58Z","timestamp":1775352238918,"version":"3.50.1"},"reference-count":95,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["031A410B"],"award-info":[{"award-number":["031A410B"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004784","name":"Novartis Stiftung f\u00fcr Medizinisch-Biologische Forschung","doi-asserted-by":"publisher","award":["21C165"],"award-info":[{"award-number":["21C165"]}],"id":[{"id":"10.13039\/501100004784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_200706"],"award-info":[{"award-number":["310030_200706"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A_175557"],"award-info":[{"award-number":["31003A_175557"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The environmental bacterium\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    causes the pneumonia Legionnaires' disease. The opportunistic pathogen forms biofilms and employs the Icm\/Dot type IV secretion system (T4SS) to replicate in amoebae and macrophages. A regulatory network comprising the\n                    <jats:italic>Legionella<\/jats:italic>\n                    quorum sensing (Lqs) system and the transcription factor LvbR controls bacterial motility, virulence and biofilm architecture. Here we show by comparative proteomics that in biofilms formed by the\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>pneumophila<\/jats:italic>\n                    \u0394\n                    <jats:italic>lqsR<\/jats:italic>\n                    or \u0394\n                    <jats:italic>lvbR<\/jats:italic>\n                    regulatory mutants the abundance of proteins encoded by a genomic \u2018fitness island\u2019, metabolic enzymes, effector proteins and flagellar components (e.g. FlaA) varies. \u2206\n                    <jats:italic>lqsR<\/jats:italic>\n                    or \u2206\n                    <jats:italic>flaA<\/jats:italic>\n                    mutants form \u2018patchy\u2019 biofilms like the parental strain JR32, while \u2206\n                    <jats:italic>lvbR<\/jats:italic>\n                    forms a \u2018mat\u2010like\u2019 biofilm.\n                    <jats:italic>Acanthamoeba castellanii<\/jats:italic>\n                    amoebae migrated more slowly through biofilms of\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>pneumophila<\/jats:italic>\n                    lacking\n                    <jats:italic>lqsR<\/jats:italic>\n                    ,\n                    <jats:italic>lvbR<\/jats:italic>\n                    ,\n                    <jats:italic>flaA<\/jats:italic>\n                    , a functional Icm\/Dot T4SS (\u2206\n                    <jats:italic>icmT<\/jats:italic>\n                    ), or secreted effector proteins. Clusters of bacteria decorated amoebae in JR32, \u2206\n                    <jats:italic>lvbR<\/jats:italic>\n                    or \u2206\n                    <jats:italic>icmT<\/jats:italic>\n                    biofilms but not in \u2206\n                    <jats:italic>lqsR<\/jats:italic>\n                    or \u2206\n                    <jats:italic>flaA<\/jats:italic>\n                    biofilms. The amoeba\u2010adherent bacteria induced promoters implicated in motility (P\n                    <jats:sub>\n                      <jats:italic>flaA<\/jats:italic>\n                    <\/jats:sub>\n                    ) or virulence (P\n                    <jats:sub>\n                      <jats:italic>sidC<\/jats:italic>\n                    <\/jats:sub>\n                    , P\n                    <jats:sub>\n                      <jats:italic>ralF<\/jats:italic>\n                    <\/jats:sub>\n                    ). Taken together, the Lqs\u2010LvbR network (quorum sensing), FlaA (motility) and the Icm\/Dot T4SS (virulence) regulate migration of\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>castellanii<\/jats:italic>\n                    through\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>pneumophila<\/jats:italic>\n                    biofilms, and \u2013 apart from the T4SS \u2013 govern bacterial cluster formation on the amoebae.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16008","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T03:04:54Z","timestamp":1649819094000},"page":"3672-3692","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Migration of\n                    <i>Acanthamoeba<\/i>\n                    through\n                    <i>Legionella<\/i>\n                    biofilms is regulated by the bacterial\n                    <scp>Lqs\u2010LvbR<\/scp>\n                    network, effector proteins and the flagellum"],"prefix":"10.1111","volume":"24","author":[{"given":"Ramon","family":"Hochstrasser","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology University of Z\u00fcrich  Gloriastrasse 30 Z\u00fcrich 8006 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Michaelis","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University of Z\u00fcrich  Gloriastrasse 30 Z\u00fcrich 8006 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Br\u00fclisauer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University of Z\u00fcrich  Gloriastrasse 30 Z\u00fcrich 8006 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Sura","sequence":"additional","affiliation":[{"name":"Institute of Microbiology University of Greifswald  Felix\u2010Hausdorff\u2010Strasse 8 Greifswald 17489 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingzhen","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University of Z\u00fcrich  Gloriastrasse 30 Z\u00fcrich 8006 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6573-1088","authenticated-orcid":false,"given":"Sandra","family":"Maa\u00df","sequence":"additional","affiliation":[{"name":"Institute of Microbiology University of Greifswald  Felix\u2010Hausdorff\u2010Strasse 8 Greifswald 17489 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f6rte","family":"Becher","sequence":"additional","affiliation":[{"name":"Institute of Microbiology University of Greifswald  Felix\u2010Hausdorff\u2010Strasse 8 Greifswald 17489 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5462-9301","authenticated-orcid":false,"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University of Z\u00fcrich  Gloriastrasse 30 Z\u00fcrich 8006 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141121660"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00038"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00610-09"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.053579"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077875"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02180-21"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac501189j"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07259.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4272-4280.2005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.508-517.2001"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.4.847-867.2000"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.04.060"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000117"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02025.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2007.06.001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-007-9006-7"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-94004-1_5"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2116-2122.2001"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02858.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01304-10"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05243.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10041-X"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12450"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0427-4"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology2041242"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2011.00247.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02645.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/008698-0"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00079"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9048-1_5"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2020.01.001"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.02370-21"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14477"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14523"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12235"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.4.1319"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100109-104034"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02449.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1967"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02889.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2015.10.001"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6826-6833.2004"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04208.x"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/000497-0"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac1031836"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046462"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2885-2895.2006"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01018-16"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.05.009"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.1.296-301.1992"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-11-3121"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067025"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1207193"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00052-09"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2016.1212988"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2010.520159"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.02.003"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2017.11.010"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-00774-0"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13021-8"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00003-12"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.67"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17657"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.12.6094-6105.2002"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003598"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.6.1658-1666.2004"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12612"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13265"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01642.x"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01054.x"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2117-2124.1999"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005307"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12258"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801929200"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.013067"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201743903"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2017.08.004"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050560"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202152972"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13246"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00061"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00405-20"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10307"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.3390\/s120302899"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01002-08"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.03.004"},{"key":"e_1_2_8_90_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02167.x"},{"key":"e_1_2_8_91_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01005.x"},{"key":"e_1_2_8_92_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01951.x"},{"key":"e_1_2_8_93_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20140251"},{"key":"e_1_2_8_94_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1145"},{"key":"e_1_2_8_95_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200794"},{"key":"e_1_2_8_96_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020046"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.16008","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1462-2920.16008","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.16008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T03:06:02Z","timestamp":1761879962000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.16008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,25]]},"references-count":95,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1111\/1462-2920.16008"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.16008","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,25]]},"assertion":[{"value":"2022-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}